New Model of Ventral Spinal Cord Lesion Induced by Balloon Compression in Rats.
New Model of Ventral Spinal Cord Lesion Induced by Balloon Compression in Rats.
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一种新的大鼠脊髓球囊压迫损伤模型。
DOI：
10.3390/biomedicines8110477
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发表时间：
2020-11-05
期刊：
影响因子：
4.7
通讯作者：
Machova Urdzikova L
中科院分区：
文献类型：
--
作者：
Krupa P;Stepankova K;Kwok JC;Fawcett JW;Cimermanova V;Jendelova P;Machova Urdzikova L
尽管目前脊髓损伤的实验模型多种多样，但人类常见的腹侧压迫性脊髓损伤模型非常有限。腹侧球囊压缩性损伤反映了人体病变的常见解剖机制，并具有通过控制球囊充气量来分级损伤严重程度的优势。在本研究中，通过椎板切除术，在T10水平前路硬膜外放置2F Fogarty导管球囊，对脊髓脊髓进行腹侧压迫。用10或15 μL生理盐水快速充气球囊，原位静置5min。通过行为和免疫组织化学测试评估病变的严重程度。体积为15 μL的压缩导致严重的运动和感觉缺陷，其表现为完全无法在水平梯子上移动，最终Basso， Beattie和Bresnahan （BBB）评分为7.4，足底测试中退缩时间缩短（11.6 s）。组织学和免疫组织化学显示白质和灰质明显缺失，运动神经元缺失，星形胶质细胞增生。当膨胀量为10 μL时，会产生轻微的瞬时缺陷。腹侧脊髓损伤没有其他球囊压缩模型。本研究提供并验证了一种新颖的、易于复制的腹侧压迫性脊髓损伤模型，该模型由Fogarty导管充气气球引入。对于严重的不完全缺陷，充气容积应保持在15 μL。
Despite the variety of experimental models of spinal cord injury (SCI) currently used, the model of the ventral compression cord injury, which is commonly seen in humans, is very limited. Ventral balloon compression injury reflects the common anatomical mechanism of a human lesion and has the advantage of grading the injury severity by controlling the inflated volume of the balloon. In this study, ventral compression of the SCI was performed by the anterior epidural placement of the balloon of a 2F Fogarty’s catheter, via laminectomy, at the level of T10. The balloon was rapidly inflated with 10 or 15 μL of saline and rested in situ for 5 min. The severity of the lesion was assessed by behavioral and immunohistochemical tests. Compression with the volume of 15 μL resulted in severe motor and sensory deficits represented by the complete inability to move across a horizontal ladder, a final Basso, Beattie and Bresnahan (BBB) score of 7.4 and a decreased withdrawal time in the plantar test (11.6 s). Histology and immunohistochemistry revealed a significant loss of white and gray matter with a loss of motoneuron, and an increased size of astrogliosis. An inflation volume of 10 μL resulted in a mild transient deficit. There are no other balloon compression models of ventral spinal cord injury. This study provided and validated a novel, easily replicable model of the ventral compression SCI, introduced by an inflated balloon of Fogarty´s catheter. For a severe incomplete deficit, an inflated volume should be maintained at 15 μL.
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影响因子：
7.5
作者：
Amemori T;Ruzicka J;Romanyuk N;Jhanwar-Uniyal M;Sykova E;Jendelova P
通讯作者：
Jendelova P
DOI：
10.1159/000446646
发表时间：
2016
期刊：
Cells, tissues, organs
影响因子：
--
作者：
Dumont CM;Margul DJ;Shea LD
通讯作者：
Shea LD
影响因子：
5.8
作者：
Ansorena, Eduardo;De Berdt, Pauline;des Rieux, Anne
通讯作者：
des Rieux, Anne
影响因子：
4.7
作者：
Krupa, Petr;Svobodova, Barbora;Urdzikova, Lucia Machova
通讯作者：
Urdzikova, Lucia Machova
DOI：
10.1523/jneurosci.1924-10.2010
发表时间：
2010-07-21
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Brock JH;Rosenzweig ES;Blesch A;Moseanko R;Havton LA;Edgerton VR;Tuszynski MH
通讯作者：
Tuszynski MH