Live imaging of whole mouse embryos during gastrulation: migration analyses of epiblast and mesodermal cells.
Live imaging of whole mouse embryos during gastrulation: migration analyses of epiblast and mesodermal cells.
复制标题
DOI：
10.1371/journal.pone.0064506
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Nonaka S
中科院分区：
文献类型：
--
作者：
Ichikawa T;Nakazato K;Keller PJ;Kajiura-Kobayashi H;Stelzer EH;Mochizuki A;Nonaka S
在小鼠胚胎的原肠胚形成过程中，细胞的动态运动包括上胚层内陷和中胚层扩张，从而形成了三层的身体结构。然而，由于实时成像的限制，这些运动的精确细节有时难以理解。为了克服这个问题，我们开发了能够用数字扫描光片显微镜（DSLM）观察活的小鼠胚胎的技术。所获得的GFP表达细胞核的深度和高时间分辨率图像以及随后的3D跟踪分析揭示了以下发现：（i）在胚胎第（E）6天和第6.5天，在外胚层中发生核间迁移（INM）。(ii)INM样迁移发生在E5.5胚胎，当外胚层是单层，尚未假复层。(iii)在E6.5处INM的主要驱动力不是来自相邻核的压力。(iv)中胚层细胞不是以片状的形式迁移，而是以没有协调的单个细胞迁移。
During gastrulation in the mouse embryo, dynamic cell movements including epiblast invagination and mesodermal layer expansion lead to the establishment of the three-layered body plan. The precise details of these movements, however, are sometimes elusive, because of the limitations in live imaging. To overcome this problem, we developed techniques to enable observation of living mouse embryos with digital scanned light sheet microscope (DSLM). The achieved deep and high time-resolution images of GFP-expressing nuclei and following 3D tracking analysis revealed the following findings: (i) Interkinetic nuclear migration (INM) occurs in the epiblast at embryonic day (E)6 and 6.5. (ii) INM-like migration occurs in the E5.5 embryo, when the epiblast is a monolayer and not yet pseudostratified. (iii) Primary driving force for INM at E6.5 is not pressure from neighboring nuclei. (iv) Mesodermal cells migrate not as a sheet but as individual cells without coordination.
登录
查看更多内容
影响因子：
64.5
作者：
Norden C;Young S;Link BA;Harris WA
通讯作者：
Harris WA
影响因子：
9.2
作者：
Arboleda-Estudillo, Yohanna;Krieg, Michael;Heisenberg, Carl-Philipp
通讯作者：
Heisenberg, Carl-Philipp
影响因子：
11.4
作者：
Kosodo, Yoichi;Suetsugu, Taeko;Suda, Masumi;Mimori-Kiyosue, Yuko;Toida, Kazunori;Baba, Shoji A.;Kimura, Akatsuki;Matsuzaki, Fumio
通讯作者：
Matsuzaki, Fumio
影响因子：
4
作者：
Nowotschin, Sonja;Hadjantonakis, Anna-Katerina
通讯作者：
Hadjantonakis, Anna-Katerina
影响因子：
64.8
作者：
FUJITA, S
通讯作者：
FUJITA, S