S-Nitrosoglutathione-Based Nitric Oxide-Releasing Nanofibers Exhibit Dual Antimicrobial and Antithrombotic Activity for Biomedical Applications.
S-Nitrosoglutathione-Based Nitric Oxide-Releasing Nanofibers Exhibit Dual Antimicrobial and Antithrombotic Activity for Biomedical Applications.
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基于 S-亚硝基谷胱甘肽的释放一氧化氮的纳米纤维在生物医学应用中表现出双重抗菌和抗血栓活性。
DOI：
10.1002/mabi.202000248
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发表时间：
2021-01
影响因子：
4.6
通讯作者：
Handa H
中科院分区：
文献类型：
--
作者：
Douglass M;Hopkins S;Pandey R;Singha P;Norman M;Handa H
纳米纤维作为血液和医疗器械之间的物理屏障的新用途允许在通常受到血栓形成，延迟愈合和慢性感染困扰的器械上进行可修改的创新表面涂层。在这项研究中，一氧化氮（NO）供体S-亚硝基谷胱甘肽（GSNO）与可生物降解的聚合物聚羟基丁酸酯（PHB）和聚乳酸（PLA）的血液相容性，抗菌纳米纤维的制造量身定制的血液接触应用共混。记录不同浓度的聚羟基丁酸酯和聚乳酸的应力/应变行为，以优化纳米纤维的机械性能。结合不同浓度的GSNO（10，15，20重量%）的纳米纤维进行了评估的基础上，其NO释放动力学。PLA/PHB + 20 wt% GSNO纳米纤维在72 h内显示出最大的NO释放（0.4-1.5 × 10−10 mol mg−1 min−1）。暴露于金黄色葡萄球菌24小时后，释放NO的纤维成功地减少了约80%的粘附细菌计数。与对照纳米纤维相比，暴露于猪血浆的NO释放纳米纤维使血小板粘附减少64.6%。发现纳米纤维对NIH/3 T3小鼠成纤维细胞无细胞毒性（>95%活力），并且DAPI和鬼笔环肽染色显示在NO释放纤维上培养的成纤维细胞具有改善的细胞粘附和功能。因此，这些新型NO释放纳米纤维为血液接触医疗器械提供了安全的抗微生物和血液相容性涂层。医疗器械使用的常见临床并发症（包括慢性感染、延迟愈合和血栓形成）对患者结局有显著影响。在这篇文章中，Douglass及其同事制造并广泛评估了释放一氧化氮的聚乳酸/聚羟基丁酸酯聚合物纳米纤维，作为改进医疗器械应用的潜在抗菌和抗血栓表面改性。
The novel use of nanofibers as a physical barrier between blood and medical devices has allowed for modifiable, innovative surface coatings on devices ordinarily plagued by thrombosis, delayed healing, and chronic infection. In this study, the nitric oxide (NO) donor S-nitrosoglutathione (GSNO) was blended with the biodegradable polymers polyhydroxybutyrate (PHB) and polylactic acid (PLA) for the fabrication of hemocompatible, antibacterial nanofibers tailored for blood-contacting applications. Stress/strain behavior of different concentrations of PHB and PLA were recorded to optimize the mechanical properties of the nanofibers. Nanofibers incorporated with different concentrations of GSNO (10, 15, 20 wt%) were evaluated based on their NO-releasing kinetics. PLA/PHB + 20 wt% GSNO nanofibers displayed the greatest NO release over 72 h (0.4–1.5 × 10−10 mol mg−1 min−1). NO-releasing fibers successfully reduced viable adhered bacterial counts by ~80% after 24 h of exposure to Staphylococcus aureus. NO-releasing nanofibers exposed to porcine plasma reduced platelet adhesion by 64.6% compared to control nanofibers. The nanofibers were found non-cytotoxic (>95% viability) towards NIH/3T3 mouse fibroblasts, and DAPI and phalloidin staining showed that fibroblasts cultured on NO-releasing fibers had improved cellular adhesion and functionality. Therefore, these novel NO-releasing nanofibers provide a safe antimicrobial and hemocompatible coating for blood-contacting medical devices. Frequent clinical complications of medical device use including chronic infection, delayed healing, and thrombosis have significant implications for patient outcome. In this article, Douglass and coworkers have fabricated and extensively evaluated nitric oxide-releasing polylactic acid/polyhydroxybutyrate polymeric nanofibers as a potential antimicrobial and antithrombotic surface modification for improved medical device applications.
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影响因子：
4.1
作者：
Colombo C;Li M;Watanabe S;Messa P;Edefonti A;Montini G;Moscatelli D;Rastaldi MP;Cellesi F
通讯作者：
Cellesi F
影响因子：
4.6
作者：
Badv M;Jaffer IH;Weitz JI;Didar TF
通讯作者：
Didar TF
影响因子：
--
作者：
Altaee N;El-Hiti GA;Fahdil A;Sudesh K;Yousif E
通讯作者：
Yousif E
DOI：
10.1016/j.ymthe.2017.06.021
发表时间：
2017-09-06
期刊：
Molecular therapy : the journal of the American Society of Gene Therapy
影响因子：
--
作者：
Howlin RP;Cathie K;Hall-Stoodley L;Cornelius V;Duignan C;Allan RN;Fernandez BO;Barraud N;Bruce KD;Jefferies J;Kelso M;Kjelleberg S;Rice SA;Rogers GB;Pink S;Smith C;Sukhtankar PS;Salib R;Legg J;Carroll M;Daniels T;Feelisch M;Stoodley P;Clarke SC;Connett G;Faust SN;Webb JS
通讯作者：
Webb JS
影响因子：
4.2
作者：
Baylón K;Rodríguez-Camarillo P;Elías-Zúñiga A;Díaz-Elizondo JA;Gilkerson R;Lozano K
通讯作者：
Lozano K