Distinct colon mucosa microbiomes associated with tubular adenomas and serrated polyps.
Distinct colon mucosa microbiomes associated with tubular adenomas and serrated polyps.
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DOI：
10.1038/s41522-022-00328-6
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发表时间：
2022-08-29
影响因子：
9.2
通讯作者：
Whiteson, Katrine L.
中科院分区：
文献类型：
--
作者：
Avelar-Barragan, Julio;DeDecker, Lauren;Lu, Zachary;Coppedge, Bretton;Karnes, William E.;Whiteson, Katrine L.
结直肠癌是世界上第二大致命癌症和第三大常见癌症。它的发展是异质性的，具有多种致癌机制。两种不同的机制包括腺瘤-癌序列和锯齿状通路。肠道微生物组已被确定为腺瘤-癌序列中的关键参与者，但其在锯齿状癌发生中的作用尚不清楚。在这项研究中，我们使用结肠粘膜和粪便样本对140名无息肉和携带息肉的个体的肠道微生物组进行了表征，以确定微生物组组成是否与两个关键途径中的每一个相关。我们发现结肠粘膜和粪便样本的微生物组之间存在显着差异，样本类型解释了微生物组中观察到的10-15%的变化。从每个个体收集多次粘膜刷拭，以研究息肉和健康肠组织之间的肠道微生物组是否存在差异，但没有发现差异。粘膜抽吸物取样显示，管状腺瘤和锯齿状息肉个体的微生物组与彼此和无息肉个体之间存在显著差异，解释了微生物组中1-4%的变异。微生物组组成还使得能够使用随机森林准确预测受试者息肉类型，其产生0.87-0.99的曲线下面积值。通过直接对结肠粘膜取样并区分结直肠癌的不同发展途径，我们的研究有助于表征锯齿状致癌作用的潜在机制靶点。这项研究还通过评估每种方法的实用性和对微生物群落组成的影响，深入了解了多种微生物组采样策略。
Colorectal cancer is the second most deadly and third most common cancer in the world. Its development is heterogenous, with multiple mechanisms of carcinogenesis. Two distinct mechanisms include the adenoma-carcinoma sequence and the serrated pathway. The gut microbiome has been identified as a key player in the adenoma-carcinoma sequence, but its role in serrated carcinogenesis is less clear. In this study, we characterized the gut microbiome of 140 polyp-free and polyp-bearing individuals using colon mucosa and fecal samples to determine if microbiome composition was associated with each of the two key pathways. We discovered significant differences between the microbiomes of colon mucosa and fecal samples, with sample type explaining 10–15% of the variation observed in the microbiome. Multiple mucosal brushings were collected from each individual to investigate whether the gut microbiome differed between polyp and healthy intestinal tissue, but no differences were found. Mucosal aspirate sampling revealed that the microbiomes of individuals with tubular adenomas and serrated polyps were significantly different from each other and polyp-free individuals, explaining 1–4% of the variance in the microbiome. Microbiome composition also enabled the accurate prediction of subject polyp types using Random Forest, which produced an area under curve values of 0.87–0.99. By directly sampling the colon mucosa and distinguishing between the different developmental pathways of colorectal cancer, our study helps characterize potential mechanistic targets for serrated carcinogenesis. This research also provides insight into multiple microbiome sampling strategies by assessing each method’s practicality and effect on microbial community composition.
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影响因子：
29
作者：
Donohoe DR;Garge N;Zhang X;Sun W;O'Connell TM;Bunger MK;Bultman SJ
通讯作者：
Bultman SJ
影响因子：
7.7
作者：
Beghini F;McIver LJ;Blanco-Míguez A;Dubois L;Asnicar F;Maharjan S;Mailyan A;Manghi P;Scholz M;Thomas AM;Valles-Colomer M;Weingart G;Zhang Y;Zolfo M;Huttenhower C;Franzosa EA;Segata N
通讯作者：
Segata N
影响因子：
3.7
作者：
Chen W;Liu F;Ling Z;Tong X;Xiang C
通讯作者：
Xiang C
影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
DOI：
10.1136/bmj.d6617
发表时间：
2011-11-10
期刊：
BMJ (Clinical research ed.)
影响因子：
--
作者：
Aune D;Chan DS;Lau R;Vieira R;Greenwood DC;Kampman E;Norat T
通讯作者：
Norat T