Oxidative Stress Is Associated with Telomere Interaction Impairment and Chromatin Condensation Defects in Spermatozoa of Infertile Males.
Oxidative Stress Is Associated with Telomere Interaction Impairment and Chromatin Condensation Defects in Spermatozoa of Infertile Males.
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氧化应激与不育男性的精子中的端粒相互作用障碍和染色质缩合缺陷有关。
DOI：
10.3390/antiox10040593
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发表时间：
2021-04-12
期刊：
影响因子：
--
通讯作者：
Rives N
中科院分区：
文献类型：
--
作者：
Berby B;Bichara C;Rives-Feraille A;Jumeau F;Pizio PD;Sétif V;Sibert L;Dumont L;Rondanino C;Rives N
端粒长度可以受到生活方式因素或环境暴露产生的活性氧（ROS）的影响。我们试图确定氧化应激是否对精子核改变有影响，特别是对不育男性精子中染色质组织和端粒相互作用的影响。我们进行了一项观察性和前瞻性研究，包括52名男性，分配在“病例组”（30名不育男性常规精液参数改变）和“对照组”（22名男性常规精液参数正常）。使用CellROX ®探针测定精子上的ROS检测。采用定量荧光原位杂交（Q-FISH）检测端粒相对长度和数目，苯胺蓝染色检测染色质凝聚，末端脱氧核苷酸转移酶dUTP缺口末端标记检测DNA片段化，FISH检测非整倍体，8-羟基-2 ′-脱氧鸟苷免疫染色检测DNA氧化损伤。与对照组相比，不育男性的细胞质活性氧水平和染色质凝聚缺陷显著增加，每个精子的端粒信号平均数也较高。此外，精子端粒信号的平均数与染色质凝聚缺陷精子的百分比呈正相关。在常规精液参数改变的不育男性中，氧化应激与端粒相互作用损伤和染色质凝聚缺陷相关。
Telomere length can be influenced by reactive oxygen species (ROS) generated by lifestyle factors or environmental exposure. We sought to determine whether oxidative stress has an impact on sperm nuclear alterations, especially on chromatin organization and telomere interactions in the spermatozoa of infertile males. We performed an observational and prospective study including fifty-two males, allocated in the “case group” (30 infertile males presenting conventional semen parameter alterations) and the “control group” (22 males with normal conventional semen parameters). ROS detection was determined on spermatozoa using CellROX© probes. Sperm nuclear damage was assessed using quantitative fluorescence in situ hybridization (Q-FISH) for relative telomere length and telomere number, aniline blue staining for chromatin condensation, terminal deoxynucleotidyl transferase dUTP nick-end labeling for DNA fragmentation, and FISH for aneuploidy and 8-hydroxy-2′-deoxyguanosine immunostaining for oxidative DNA damages. Infertile males had significantly increased levels of cytoplasmic ROS and chromatin condensation defects as well as a higher mean number of telomere signals per spermatozoon in comparison with controls. In addition, the mean number of sperm telomere signals were positively correlated with the percentage of spermatozoa with chromatin condensation defect. In infertile males with conventional semen parameter alterations, oxidative stress is associated with telomere interaction impairment and chromatin condensation defects.
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影响因子：
4
作者：
Aston KI;Hunt SC;Susser E;Kimura M;Factor-Litvak P;Carrell D;Aviv A
通讯作者：
Aviv A
影响因子：
4.6
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3.6
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6.7
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Ko, Edmund Y.;Sabanegh, Edmund S., Jr.;Agarwal, Ashok
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Agarwal, Ashok