Nkx6.1 controls migration and axon pathfinding of cranial branchio-motoneurons
Nkx6.1 controls migration and axon pathfinding of cranial branchio-motoneurons
复制标题
Nkx6.1 控制颅鳃运动神经元的迁移和轴突寻路
作者：
Myriam Müller;N. Jabs;Dietrich E. Lork;Bernd Fritzsch;M. Sander
由于许多研究都集中在运动神经元的指定机制上，对控制有丝分裂后运动神经元后续发育的因素知之甚少。此前，我们曾证明转录因子Nkx6.1是脊髓中体细胞运动神经元前体细胞早期指定所必需的。我们目前对Nkx6.1缺陷小鼠胚胎的后脑运动神经元发育的分析表明，早期的分支运动神经元的规范与Nkx6.1的功能无关，但它是它们随后发育所必需的。在Nkx6.1突变小鼠中，我们观察到迁移以及分支运动神经元轴突投射方面的缺陷。对面部分支运动神经元迁移缺陷的详细分析表明，细胞表面受体Ret和Unc5h3在迁移前神经元中异位表达，但在菱形环境中没有变化。综上所述，我们的发现表明Nkx6.1在有丝分裂后运动神经元的发育中是必需的，并表明细胞自主功能在控制鳃-运动神经元迁移中的作用。
As many studies have focused on the mechanisms of motoneuron specification, little is known about the factors that control the subsequent development of postmitotic motoneurons. Previously, we showed that the transcription factor Nkx6.1 is required for the early specification of somatic motoneuron progenitors in the spinal cord. Our present analysis of hindbrain motoneuron development in Nkx6.1-deficient mouse embryos reveals that the early specification of branchio-motoneurons is independent of Nkx6.1 function, but that it is required for their subsequent development. In Nkx6.1 mutant mice, we observed defects in the migration, as well as in the axon projections of branchio-motoneurons. A detailed analysis of the migratory defect in facial branchio-motoneurons reveals ectopic expression of the cell surface receptors Ret and Unc5h3 in premigratory neurons, but no changes in the rhombomeric environment. Taken together, our findings demonstrate a requirement for Nkx6.1 in the development of postmitotic motoneurons, and suggest a cell-autonomous function in the control of branchio-motoneuron migration.
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影响因子：
4.6
作者：
H. Enomoto;Peter A. Crawford;A. Gorodinsky;R. Heuckeroth;Eugene M. Johnson;J. Milbrandt
通讯作者：
H. Enomoto;Peter A. Crawford;A. Gorodinsky;R. Heuckeroth;Eugene M. Johnson;J. Milbrandt
影响因子：
4.6
作者：
V. Pachnis;B. Mankoo;F. Costantini
通讯作者：
V. Pachnis;B. Mankoo;F. Costantini
靶向破坏 Hoxb-1 的小鼠无法形成第七神经的运动核。
DOI：
10.1242/dev.122.10.3217
发表时间：
1996
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Goddard,JM;Rossel,M;Manley,NR;Capecchi,MR
通讯作者：
Capecchi,MR
影响因子：
10.5
作者：
Sander, M;Paydar, S;Rubenstein, JLR
通讯作者：
Rubenstein, JLR
影响因子：
2.7
作者：
Cooper, KL;Leisenring, WM;Moens, CB
通讯作者：
Moens, CB