Maternal bisphenol a exposure promotes the development of experimental asthma in mouse pups.
Maternal bisphenol a exposure promotes the development of experimental asthma in mouse pups.
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DOI：
10.1289/ehp.0901259
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发表时间：
2010-02
影响因子：
10.4
通讯作者：
Goldblum RM
中科院分区：
文献类型：
--
作者：
Midoro-Horiuti T;Tiwari R;Watson CS;Goldblum RM
我们最近报道，各种环境雌激素诱导肥大细胞脱颗粒和增强IgE介导的过敏介质在体外释放。我们假设环境雌激素会增强过敏性致敏以及支气管炎症和反应性。为了验证这一假设，我们通过母体负荷将胎儿和新生小鼠暴露于常见的环境雌激素双酚A（BPA），并评估幼仔对过敏致敏和支气管激发的反应。雌性BALB/c小鼠从受孕前1周至研究结束，在其饮用水中接受10 μg/mL BPA。新生小鼠在出生后第4天单次腹腔内给予5 μg卵清蛋白（OVA）和氢氧化铝，并在第13、14和15天通过雾化吸入3% OVA 10 min。最后一次雾化后48小时，我们通过酶联免疫吸附试验（ELISA）和计数支气管肺泡灌洗液中的嗜酸性粒细胞，全身气压体积描记法和强迫振荡技术来评估血清IgE抗体对OVA和气道炎症和高反应性。与未暴露的母亲相比，BPA暴露母亲的新生儿对这种“次优”致敏反应具有更高的血清IgE抗OVA浓度（p < 0.05），并且其气道中的嗜酸性粒细胞炎症显著更大。BPA处理母亲的OVA致敏新生儿的气道反应性比未暴露母亲的新生儿的气道反应性增强（p < 0.05）。围产期暴露于BPA可增强哮喘易感动物模型的过敏敏感性和支气管炎症及反应性。
We recently reported that various environmental estrogens induce mast cell degranulation and enhance IgE-mediated release of allergic mediators in vitro. We hypothesized that environmental estrogens would enhance allergic sensitization as well as bronchial inflammation and responsiveness. To test this hypothesis, we exposed fetal and neonatal mice to the common environmental estrogen bisphenol A (BPA) via maternal loading and assessed the pups’ response to allergic sensitization and bronchial challenge. Female BALB/c mice received 10 μg/mL BPA in their drinking water from 1 week before impregnation to the end of the study. Neonatal mice were given a single 5 μg intraperitoneal dose of ovalbumin (OVA) with aluminum hydroxide on postnatal day 4 and 3% OVA by nebulization for 10 min on days 13, 14, and 15. Forty-eight hours after the last nebulization, we assessed serum IgE antibodies to OVA by enzyme-linked immunosorbent assay (ELISA) and airway inflammation and hyperresponsiveness by enumerating eosinophils in bronchoalveolar lavage fluid, whole-body barometric plethysmography, and a forced oscillation technique. Neonates from BPA-exposed mothers responded to this “suboptimal” sensitization with higher serum IgE anti-OVA concentrations compared with those from unexposed mothers (p < 0.05), and eosinophilic inflammation in their airways was significantly greater. Airway responsiveness of the OVA-sensitized neonates from BPA-treated mothers was enhanced compared with those from unexposed mothers (p < 0.05). Perinatal exposure to BPA enhances allergic sensitization and bronchial inflammation and responsiveness in a susceptible animal model of asthma.
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影响因子：
6.1
作者：
Johnson, CC;Ownby, DR;Peterson, EL
通讯作者：
Peterson, EL
影响因子：
10.4
作者：
Narita S;Goldblum RM;Watson CS;Brooks EG;Estes DM;Curran EM;Midoro-Horiuti T
通讯作者：
Midoro-Horiuti T
影响因子：
3.3
作者：
Fernandez, M. F.;Arrebola, J. P.;Olea, N.
通讯作者：
Olea, N.
DOI：
10.1016/j.bbrc.2003.10.135
发表时间：
2003-12-12
影响因子：
3.1
作者：
Kim, YH;Kim, CS;Yang, MH
通讯作者：
Yang, MH
影响因子：
4.4
作者：
Iwata, M;Eshima, Y;Miyaura, H
通讯作者：
Miyaura, H