Low nitrogen fertilization adapts rice root microbiome to low nutrient environment by changing biogeochemical functions.
Low nitrogen fertilization adapts rice root microbiome to low nutrient environment by changing biogeochemical functions.
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DOI：
10.1264/jsme2.me13110
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发表时间：
2014
影响因子：
2.2
通讯作者：
Minamisawa K
中科院分区：
文献类型：
--
作者：
Ikeda S;Sasaki K;Okubo T;Yamashita A;Terasawa K;Bao Z;Liu D;Watanabe T;Murase J;Asakawa S;Eda S;Mitsui H;Sato T;Minamisawa K
减少肥料使用是追求可持续农业的田间管理目标之一。在这里，我们报告了低（LN），标准（SN）和高（HN）水平氮肥施用（分别为0，30和300 kg N ha-1）的水稻生态系统中细菌群落的变化。在实验之前，LN田地已经5年没有施用氮肥了。LN和HN植物与SN植物生物量相比，生物量分别减少50%和增加60%。16 S rRNA基因的分析表明LN和SN根微生物组之间的细菌群落的变化，这是由宏基因组分析在统计学上证实。伯克霍尔德氏菌属、慢生根瘤菌属和甲基弯菌属的相对丰度在LN田的根微生物组中相对于SN田显著增加。相反，产甲烷古菌的丰度在LN领域相对于SN领域减少。甲烷氧化（pmo和mmo）和植物关联（acdS和iaaMH）的功能基因在LN根微生物组中显着丰富。pmoA/mcrA基因的定量PCR和13 C甲烷实验提供了LN田水稻根系中更活跃的甲烷氧化的证据。此外，N，S，Fe和芳香族化合物的代谢功能基因在LN根微生物组中更丰富。这些结果表明，低N肥管理是一个重要因素，在塑造微生物群落结构，包括植物协会和生态地球化学过程中的关键微生物。
Reduced fertilizer usage is one of the objectives of field management in the pursuit of sustainable agriculture. Here, we report on shifts of bacterial communities in paddy rice ecosystems with low (LN), standard (SN), and high (HN) levels of N fertilizer application (0, 30, and 300 kg N ha−1, respectively). The LN field had received no N fertilizer for 5 years prior to the experiment. The LN and HN plants showed a 50% decrease and a 60% increase in biomass compared with the SN plant biomass, respectively. Analyses of 16S rRNA genes suggested shifts of bacterial communities between the LN and SN root microbiomes, which were statistically confirmed by metagenome analyses. The relative abundances of Burkholderia, Bradyrhizobium and Methylosinus were significantly increased in root microbiome of the LN field relative to the SN field. Conversely, the abundance of methanogenic archaea was reduced in the LN field relative to the SN field. The functional genes for methane oxidation (pmo and mmo) and plant association (acdS and iaaMH) were significantly abundant in the LN root microbiome. Quantitative PCR of pmoA/mcrA genes and a 13C methane experiment provided evidence of more active methane oxidation in the rice roots of the LN field. In addition, functional genes for the metabolism of N, S, Fe, and aromatic compounds were more abundant in the LN root microbiome. These results suggest that low-N-fertilizer management is an important factor in shaping the microbial community structure containing key microbes for plant associations and biogeochemical processes in paddy rice ecosystems.
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影响因子：
4.2
作者：
Hardoim PR;Andreote FD;Reinhold-Hurek B;Sessitsch A;van Overbeek LS;van Elsas JD
通讯作者：
van Elsas JD
影响因子：
4.4
作者：
Kolb, S;Knief, C;Conrad, R
通讯作者：
Conrad, R
影响因子：
2.2
作者：
Dianou D;Ueno C;Ogiso T;Kimura M;Asakawa S
通讯作者：
Asakawa S
通过改善中国集约农业系统的氮肥管理来降低环境风险
DOI：
10.1073/pnas.0813417106
发表时间：
2009-03-03
影响因子：
11.1
作者：
Ju, Xiao-Tang;Xing, Guang-Xi;Zhang, Fu-Suo
通讯作者：
Zhang, Fu-Suo
影响因子：
64.8
作者：
Bodelier, PLE;Roslev, P;Frenzel, P
通讯作者：
Frenzel, P