Development, ultrastructural pathology, and taxonomic revision of the Microsporidial genus, Pseudoloma and its type species Pseudoloma neurophilia, in skeletal muscle and nervous tissue of experimentally infected zebrafish Danio rerio.
Development, ultrastructural pathology, and taxonomic revision of the Microsporidial genus, Pseudoloma and its type species Pseudoloma neurophilia, in skeletal muscle and nervous tissue of experimentally infected zebrafish Danio rerio.
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DOI：
10.1111/j.1550-7408.2011.00591.x
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发表时间：
2012-01
期刊：
影响因子：
--
通讯作者：
Takvorian PM
中科院分区：
文献类型：
--
作者：
Cali A;Kent M;Sanders J;Pau C;Takvorian PM
嗜神经假瘤小孢子虫最初被报道感染斑马鱼的中枢神经系统，引起前凸并最终死亡。随后，肌肉组织感染也被确认。为了了解这种微孢子虫的感染过程、发育和超微结构病理，我们给幼鱼和成年斑马鱼喂食嗜神经孢子虫孢子。暴露3 h后，在幼鱼消化道中检测到孢子。到第4.5天(d)，在肌肉组织中鉴定出寄生虫的发育阶段。第8天收集的幼虫湿法标本显示，脊索附近的脊髓内有孢子聚集体。所有阶段的寄生虫，包括孢子，都存在于幼鱼8 d的肌肉组织中。术后45 d处死的成年斑马鱼神经完全感染。对嗜神经线虫发育周期的超微结构研究表明，增殖阶段经历核分裂，产生四核阶段，然后分裂成单核细胞。增生细胞的质膜有一个先前未报道的糖萼样被膜，它与宿主细胞质相连接。产孢期形成孢子囊液泡（SPOV），起源于质乳致密的表面外壳，从孢子体上“起泡”。单核孢子母细胞和孢子在SPOV中发育。神经和肌肉的发育周期是相同的。SPOV表面相对较厚，是最外层的寄生虫表面实体；因此不会形成异种瘤。根据本研究提供的新信息，修改了pseudooloma属及其模式种P. neurophilia的分类描述，并描述了其生命周期。
The microsporidium Pseudoloma neurophilia was initially reported to infect the central nervous system of zebrafish causing lordosis and eventually death. Subsequently, muscle tissue infections were also identified. To understand the infection process, development, and ultrastructural pathology of this microsporidium, larval and adult zebrafish were fed P. neurophilia spores. Spores were detected in the larval fish digestive tract 3 h post-exposure (PE). By 4.5 days (d) PE, developing parasite stages were identified in muscle tissue. Wet preparations of larvae collected at 8 d PE showed aggregates of spores in the spinal cord adjacent to the notochord. All parasite stages, including spores, were present in the musculature of larval fish 8 d PE. Adult zebrafish sacrificed 45 d PE had fully developed infections in nerves. Ultrastructural study of the developmental cycle of P. neurophilia revealed that proliferative stages undergo karyokinesis, producing tetranucleate stages that then divide into uninucleate cells. The plasmalemma of proliferative cells has a previously unreported glycocalyx-like coat that interfaces with the host cell cytoplasm. Sporogonic stages form sporophorous vacuoles (SPOV) derived from the plasmalemmal dense surface coat, which “blisters” off sporonts. Uninucleate sporoblasts and spores develop in the SPOV. The developmental cycle is identical in both nerve and muscle. The SPOV surface is relatively thick and is the outermost parasite surface entity; thus xenomas are not formed. Based on the new information provided by this study, the taxonomic description of the genus Pseudoloma and its type species, P. neurophilia is modified and its life cycle described.
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影响因子：
1.6
作者：
Kent, ML;Speare, DJ
通讯作者：
Speare, DJ
影响因子：
2
作者：
S치nchez, JG;Speare, DJ;Jones, SRM
通讯作者：
Jones, SRM
影响因子：
2.5
作者：
Kent, ML;Bishop-Stewart, JK
通讯作者：
Bishop-Stewart, JK
影响因子：
2.2
作者：
Matthews, JL;Brown, AMV;Kent, ML
通讯作者：
Kent, ML
影响因子：
1.4
作者：
Kent, Michael L.;Buchner, Cari;Tanguay, Robert L.
通讯作者：
Tanguay, Robert L.