Specific binding of basic fibroblast growth factor to basement membrane‐like structures and to purified heparan sulfate proteoglycan of the EHS tumor
Specific binding of basic fibroblast growth factor to basement membrane‐like structures and to purified heparan sulfate proteoglycan of the EHS tumor
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碱性成纤维细胞生长因子与 EHS 肿瘤的基底膜样结构和纯化的硫酸乙酰肝素蛋白聚糖的特异性结合
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发表时间：
1988
影响因子：
5.6
通讯作者：
Y. Courtois
中科院分区：
文献类型：
--
作者：
M. Vigny;M. Ollier;M. Lavigne;N. Fayein;J. ;M. Laurent;Y. Courtois
使用不同技术研究了碘化碱性成纤维细胞生长因子（bFGF）与从Engelbreth霍尔姆群（EHS）肉瘤纯化的低密度硫酸乙酰肝素蛋白聚糖的结合。该肿瘤明显含有bFGF，其水平与在其他组织如人脑或牛脑中发现的水平相当。放射自显影显示125 I bFGF与EHS冷冻切片的基底膜样基质强烈结合。碘化碱性成纤维细胞生长因子结合纯化的硫酸乙酰肝素蛋白聚糖，但不层粘连蛋白或IV型胶原蛋白，从同一肿瘤分离的三个组件。相反，酸性成纤维细胞生长因子（aFGF）显示可忽略不计的硫酸乙酰肝素蛋白聚糖的结合。结合的碱性成纤维细胞生长因子的冷冻切片和纯化的蛋白多糖可以强烈抑制肝素和被取代的过量的未标记的因子和肝素酶和肝素酶治疗后完全抑制。结合是盐浓度的函数，在0.6 M NaCl时被消除。Scatchard分析表明亲和位点具有约30 nM的Kd，该值比Moscatelli最近报道的值高10-15（J. Cell.生理学，131：123-130，1987），在幼仓鼠肾（BHK）细胞表面上存在低亲和力结合位点的情况下。
The binding of iodinated basic fibroblast growth factor (bFGF) to low‐density heparan sulfate proteoglycan purified from the Engelbreth Holm Swarm (EHS) sarcoma was investigated using different techniques. The tumor clearly contained bFGF, the level being comparable to that found in other tissues such as human or bovine brain. 125I bFGF strongly bound to the basement membrane‐like matrix of EHS frozen sections as revealed by autoradiography. Iodinated bFGF bound to purified heparan sulfate proteoglycan but not to laminin or collagen type IV, three components isolated from the same tumor. In contrast, acidic fibroblast growth factor (aFGF) displayed negligible binding to heparan sulfate proteoglycan. Binding of bFGF to frozen sections and to purified proteoglycan could be strongly inhibited by heparin and was displaced by an excess of unlabeled factor and completely suppressed after heparitinase and heparinase treatments. Binding was a function of the salt concentration and was abolished at 0.6 M NaCl. Scatchard analysis indicated the affinity site had a Kd of about 30 nM, a value 10–15 higher than that recently reported by Moscatelli (J. Cell. Physiol., 131:123–130, 1987) in the case of the low‐affinity binding sites present on the surface of baby hamster kidney (BHK) cells.
影响因子：
56.9
作者：
THORNTON, SC;MUELLER, SN;LEVINE, EM
通讯作者：
LEVINE, EM
影响因子：
56.9
作者：
SHING, Y;FOLKMAN, J;KLAGSBRUN, M
通讯作者：
KLAGSBRUN, M