Impact of periodontal therapy on the subgingival microbiota of severe periodontitis: comparison between good responders and individuals with refractory periodontitis using the human oral microbe identification microarray.
Impact of periodontal therapy on the subgingival microbiota of severe periodontitis: comparison between good responders and individuals with refractory periodontitis using the human oral microbe identification microarray.
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DOI：
10.1902/jop.2012.110566
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发表时间：
2012-10
影响因子：
4.3
通讯作者：
Paster BJ
中科院分区：
文献类型：
--
作者：
Colombo AP;Bennet S;Cotton SL;Goodson JM;Kent R;Haffajee AD;Socransky SS;Hasturk H;Van Dyke TE;Dewhirst FE;Paster BJ
本研究应用人类口腔微生物鉴定芯片（HOMIM）比较了难治性牙周炎（RP）和可治疗性牙周炎（GR）患者牙周治疗前后龈下菌群的变化。根据平均附着丧失（AL）和/或洁治、根面平整、手术和全身应用阿莫西林和甲硝唑后AL ≥2.5 mm的≥ 3个位点将慢性牙周炎患者分为RP（n=17）或GR（n=30），根据平均附着获得和治疗后AL ≥2.5 mm的无位点分为GR。在基线和治疗后15个月采集龈下菌斑样本，并通过HOMIM分析分析300种菌的存在。使用Wilcoxon检验寻求治疗前后分类群的显著差异。治疗后，两组中评价的大多数种属的患病率均下降;然而，只有一小部分微生物受到显著影响。在RP中以高频率增加或持续但在GR中显著降低的物种包括拟杆菌属，牙髓卟啉单胞菌，牙龈卟啉单胞菌，普氏菌属，Tannerella pasthia，Dialister spp.月形单胞菌属，卡托内氏菌，真杆菌属，嗜酸纤维素、微小单胞菌、消化链霉菌属OT 113、梭杆菌属OT 203、解乳假杆菌、中间链球菌或星座链球菌和卫星梭菌。相比之下，生痰二氧化碳嗜纤维菌、人心杆菌、溶血双生菌、副流感嗜血杆菌、口腔金杆菌、奇异劳托菌、细长奈瑟氏菌、龋齿罗氏菌、南方链球菌和韦荣球菌属。与治疗的成功更相关。持续存在的假定和新的牙周病原体，以及低患病率的有益物种与慢性“难治性”牙周炎。
This study compared the changes on the subgingival microbiota of subjects with “refractory” periodontitis (RP) or treatable periodontitis (GR) before and after periodontal therapy by using the Human Oral Microbe Identification Microarray (HOMIM). Individuals with chronic periodontitis were classified as RP (n=17) based on mean attachment loss (AL) and/or >3 sites with AL ≥2.5 mm after scaling and root planing, surgery and systemically administered amoxicillin and metronidazole or as GR (n=30) based on mean attachment gain and no sites with AL ≥2.5 mm after treatment. Subgingival plaque samples were taken at baseline and 15 months after treatment and analyzed for the presence of 300 species by HOMIM analysis. Significant differences in taxa before and after therapy were sought using the Wilcoxon test. The majority of species evaluated decreased in prevalence in both groups after treatment; however, only a small subset of organisms was significantly affected. Species that increased or persisted in high frequency in RP but were significantly reduced in GR included Bacteroidetes sp., Porphyromonas endodontalis, Porphyromonas gingivalis, Prevotella spp., Tannerella forsythia, Dialister spp., Selenomonas spp., Catonella morbi, Eubacterium spp., Filifactor alocis, Parvimonas micra, Peptostreptococcus sp. OT113, Fusobacterium sp. OT203, Pseudoramibacter alactolyticus, Streptococcus intermedius or Streptococcus constellatus and Shuttlesworthia satelles. In contrast, Capnocytophaga sputigena, Cardiobacterium hominis, Gemella haemolysans, Haemophilus parainfluenzae, Kingella oralis, Lautropia mirabilis, Neisseria elongata, Rothia dentocariosa, Streptococcus australis and Veillonella spp. were more associated with therapeutic success. Persistence of putative and novel periodontal pathogens, as well as low prevalence of beneficial species was associated with chronic “refractory” periodontitis.
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影响因子：
6.7
作者：
Berglundh, T;Krok, L;Lindhe, J
通讯作者：
Lindhe, J
影响因子：
6.7
作者：
Haffajee, AD;Uzel, NG;Socransky, SS
通讯作者：
Socransky, SS
影响因子：
6.7
作者：
HAFFAJEE, AD;SOCRANSKY, SS;EBERSOLE, JL
通讯作者：
EBERSOLE, JL
影响因子：
4.3
作者：
COLLINS, JG;OFFENBACHER, S;ARNOLD, RR
通讯作者：
ARNOLD, RR
影响因子：
6.7
作者：
Colombo, AP;Haffajee, AD;Socransky, SS
通讯作者：
Socransky, SS