Profiling development of abdominal organs in the pig.
Profiling development of abdominal organs in the pig.
复制标题
猪中腹部器官的发展。
DOI：
10.1038/s41598-022-19960-5
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发表时间：
2022-09-28
影响因子：
4.6
通讯作者：
Lo, Cecilia W.
中科院分区：
文献类型：
--
作者：
Gabriel, George C.;Devine, William A.;Redel, Bethany K.;Whitworth, Kristin M.;Samuel, Melissa;Spate, Lee D.;Cecil, Raissa F.;Prather, Randall S.;Wu, Yijen L.;Wells, Kevin D.;Lo, Cecilia W.
猪是研究人类发育和疾病的理想模型系统，因为它与人类的解剖、生理、大小和基因组相似。此外，CRISPR基因编辑技术的进步使基因工程猪成为研究人类病理和先天性异常的可行模型。然而，一个详细的图集说明猪的发展是必要的，以确定和建模发育缺陷。在这里，我们描述了猪腹部系统的正常发育，并展示了CRISPR基因编辑的SAP130突变猪可能出现的先天性缺陷的例子。对20天（D20）至足月不同胎龄的正常猪进行检查，并利用共聚焦显微镜、磁共振成像（MRI）和尸检对腹部器官的三维组织学重建进行研究。这显示在D20时出现了突出的中肾，这是一种仅在胚胎发生期间出现的短暂胚胎器官，而在D26时出现了将形成永久肾脏的发育中的后肾。到D64时，中肾消失，只剩下后肾。D20观察肝脏和胰腺的形成，D30和D35分别观察肝脏和胰腺的形成。脾和肾上腺首先在D26发现，到D42完成。发育中的肠和性腺在D20处可见。肠在D42时完全旋转，男性睾丸在D64时下降。该图谱和所使用的方法是识别猪不同发育阶段腹部器官发育病理的极好工具。
The pig is an ideal model system for studying human development and disease due to its similarities to human anatomy, physiology, size, and genome. Further, advances in CRISPR gene editing have made genetically engineered pigs viable models for the study of human pathologies and congenital anomalies. However, a detailed atlas illustrating pig development is necessary for identifying and modeling developmental defects. Here we describe normal development of the pig abdominal system and show examples of congenital defects that can arise in CRISPR gene edited SAP130 mutant pigs. Normal pigs at different gestational ages from day 20 (D20) to term were examined and the configuration of the abdominal organs was studied using 3D histological reconstructions with episcopic confocal microscopy, magnetic resonance imaging (MRI) and necropsy. This revealed prominent mesonephros, a transient embryonic organ present only during embryogenesis, at D20, while the developing metanephros that will form the permanent kidney are noted at D26. By D64 the mesonephroi are absent and only the metanephroi remain. The formation of the liver and pancreas was observed by D20 and complete by D30 and D35 respectively. The spleen and adrenal glands are first identified at D26 and completed by D42. The developing bowel and the gonads are identified at D20. The bowel appears completely rotated by D42, and testes in the male were descended at D64. This atlas and the methods used are excellent tools for identifying developmental pathologies of the abdominal organs in the pig at different stages of development.
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影响因子：
30.8
作者：
Liu X;Yagi H;Saeed S;Bais AS;Gabriel GC;Chen Z;Peterson KA;Li Y;Schwartz MC;Reynolds WT;Saydmohammed M;Gibbs B;Wu Y;Devine W;Chatterjee B;Klena NT;Kostka D;de Mesy Bentley KL;Ganapathiraju MK;Dexheimer P;Leatherbury L;Khalifa O;Bhagat A;Zahid M;Pu W;Watkins S;Grossfeld P;Murray SA;Porter GA Jr;Tsang M;Martin LJ;Benson DW;Aronow BJ;Lo CW
通讯作者：
Lo CW
DOI：
10.1097/00000433-200112000-00015
发表时间：
2001-12-01
影响因子：
1
作者：
Horn, KD;Devine, WA
通讯作者：
Devine, WA
DOI：
10.1007/bf00493025
发表时间：
1981-01-01
期刊：
HISTOCHEMISTRY
影响因子：
--
作者：
NEISS, WF;KLEHN, KL
通讯作者：
KLEHN, KL
影响因子：
5.7
作者：
Jacobs, RE;Ahrens, ET;Laidlaw, D
通讯作者：
Laidlaw, D
影响因子：
5.4
作者：
Gabriel GC;Devine W;Redel BK;Whitworth KM;Samuel M;Spate LD;Cecil RF;Prather RS;Wu Y;Wells KD;Lo CW
通讯作者：
Lo CW