Biological activity and magnetic resonance imaging of superparamagnetic iron oxide nanoparticles-labeled adipose-derived stem cells.
Biological activity and magnetic resonance imaging of superparamagnetic iron oxide nanoparticles-labeled adipose-derived stem cells.
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超顺磁性氧化铁纳米粒子标记的脂肪干细胞的生物活性和磁共振成像。
DOI：
10.1186/scrt191
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发表时间：
2013-04-23
影响因子：
7.5
通讯作者：
Zhang M
中科院分区：
文献类型：
--
作者：
Fan J;Tan Y;Jie L;Wu X;Yu R;Zhang M
No comparative study of adipose-derived stem cells (ADSCs) and bone marrow mesenchymal stem cells (BMSCs) by using superparamagnetic iron oxide nanoparticles (SPIOs)-labeling and magnetic resonance imaging (MRI) has been performed. We studied the biological activity and MRI of ADSCs by labeling them with SPIOs and comparing them with BMSCs. After incubating the cells in culture medium with different levels of SPIOs (control group: 0 μg/ml; Groups 1 to 3: 25, 50, and 100 μg/ml) for 24 hours, we compared ADSCs with BMSCs in terms of intracellular iron content, labeling efficiency, and cell viability. Stem cells in the culture medium containing 50 μg/ml SPIOs were induced into osteoblasts and fat cells. Adipogenic and osteogenic differentiation potentials were compared. R2* values of MRI in vitro were compared. The results showed that labeling efficiency was highest in Group 2. Intracellular iron content and R2* values increased with increasing concentrations of SPIOs, whereas cell viability decreased with increasing concentrations of SPIOs, and adipogenic and osteogenic differentiation potentials decreased. However, we found no significant difference between the two kinds of cells for any of these indexes. ADSCs can be labeled and traced as easily as BMSCs in vitro. Given their abundance and higher proliferative capacity, as was previously shown, ADSCs may be better suited to stem cell therapy than are BMSCs.
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影响因子：
3.7
作者：
van Buul GM;Kotek G;Wielopolski PA;Farrell E;Bos PK;Weinans H;Grohnert AU;Jahr H;Verhaar JA;Krestin GP;van Osch GJ;Bernsen MR
通讯作者：
Bernsen MR
影响因子：
2.9
作者：
Pawelczyk, Edyta;Arbab, Ali S.;Frank, Joseph A.
通讯作者：
Frank, Joseph A.
影响因子：
5.2
作者：
Mitchell, James B.;McIntosh, Kevin;Gimble, Jeffrey M.
通讯作者：
Gimble, Jeffrey M.
影响因子：
5.2
作者：
Kern, Susanne;Eichler, Hermann;Bieback, Karen
通讯作者：
Bieback, Karen
影响因子：
3.8
作者：
Chen, Ying-Chun;Hsiao, Jong-Kai;Huang, Dong-Ming
通讯作者：
Huang, Dong-Ming