A single-day mouse mesenteric lymph surgery in mice: an updated approach to study dietary lipid absorption, chylomicron secretion, and lymphocyte dynamics.
A single-day mouse mesenteric lymph surgery in mice: an updated approach to study dietary lipid absorption, chylomicron secretion, and lymphocyte dynamics.
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DOI：
10.1016/j.jlr.2022.100284
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发表时间：
2022-11
影响因子：
6.5
通讯作者：
Kohan, Alison B.
中科院分区：
文献类型：
--
作者：
Dedousis, Nikolaos;Teng, Lihong;Kanshana, Jitendra S.;Kohan, Alison B.
肠道通过其吸收膳食脂肪的独特功能在调节全身脂质代谢中起着至关重要的作用。在小肠中，吸收性上皮细胞在将疏水性膳食甘油三酯（TAGs）以乳糜微粒的形式分泌到肠系膜淋巴管之前，先将其乳化。除了短链和中链脂肪酸可直接从肠腔吸收进入门静脉血管系统外，动物吸收膳食TAG的唯一途径是通过乳糜微粒/肠系膜淋巴途径。由于餐后淋巴在外周血液中被稀释，在体内分离肠道脂蛋白（包括乳糜微粒）极其困难。此外，一旦餐后淋巴进入循环，乳糜微粒TAG会迅速水解。为了更好地分离大量纯的餐后乳糜微粒，我们改进了Tso团队在大鼠中高度可重复的金标准双插管技术，使其能够在小鼠中进行单日手术和淋巴采集。我们的技术比传统的2日手术模型具有显著更高的存活率，并且能够采集到超过400 μl的乳糜样淋巴，其中餐后TAG浓度较高。利用这种方法，我们发现十二指肠内给予脂质团注后，肠系膜淋巴中含有可通过流式细胞术定量的初始CD4 + T细胞群。总之，这种实验方法是一种用于确定膳食脂质吸收、肠道脂蛋白动态以及肠系膜免疫的定量工具。我们的模型也可能是研究抗原、微生物组、药代动力学以及膳食化合物吸收的有力工具。
The intestine plays a crucial role in regulating whole-body lipid metabolism through its unique function of absorbing dietary fat. In the small intestine, absorptive epithelial cells emulsify hydrophobic dietary triglycerides (TAGs) prior to secreting them into mesenteric lymphatic vessels as chylomicrons. Except for short- and medium-chain fatty acids, which are directly absorbed from the intestinal lumen into portal vasculature, the only way for an animal to absorb dietary TAG is through the chylomicron/mesenteric lymphatic pathway. Isolating intestinal lipoproteins, including chylomicrons, is extremely difficult in vivo because of the dilution of postprandial lymph in the peripheral blood. In addition, once postprandial lymph enters the circulation, chylomicron TAGs are rapidly hydrolyzed. To enhance isolation of large quantities of pure postprandial chylomicrons, we have modified the Tso group’s highly reproducible gold-standard double-cannulation technique in rats to enable single-day surgery and lymph collection in mice. Our technique has a significantly higher survival rate than the traditional 2-day surgical model and allows for the collection of greater than 400 μl of chylous lymph with high postprandial TAG concentrations. Using this approach, we show that after an intraduodenal lipid bolus, the mesenteric lymph contains naïve CD4+ T-cell populations that can be quantified by flow cytometry. In conclusion, this experimental approach represents a quantitative tool for determining dietary lipid absorption, intestinal lipoprotein dynamics, and mesenteric immunity. Our model may also be a powerful tool for studies of antigens, the microbiome, pharmacokinetics, and dietary compound absorption.
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影响因子：
17.1
作者：
Balmer, Maria L.;Slack, Emma;Macpherson, Andrew J.
通讯作者：
Macpherson, Andrew J.
影响因子：
6.5
作者：
Gao, Yu;Nelson, David W.;Yen, Chi-Liang Eric
通讯作者：
Yen, Chi-Liang Eric
影响因子：
3.7
作者：
GALKOWSKA, H;OLSZEWSKI, WL
通讯作者：
OLSZEWSKI, WL
影响因子：
9.8
作者：
Guo, QS;Avramoglu, RK;Adeli, K
通讯作者：
Adeli, K
影响因子：
2.4
作者：
Anzai K;Fukagawa K;Iwakiri R;Fujimoto K;Akashi K;Tso P
通讯作者：
Tso P