Characterization of methylation profiles in spontaneous preterm birth placental villous tissue.
Characterization of methylation profiles in spontaneous preterm birth placental villous tissue.
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DOI：
10.1371/journal.pone.0279991
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发表时间：
2023
期刊：
影响因子：
3.7
通讯作者：
Jones, Helen N.
中科院分区：
文献类型：
--
作者：
Brockway, Heather M.;Wilson, Samantha L.;Kallapur, Suhas G.;Buhimschi, Catalin S.;Muglia, Louis J.;Jones, Helen N.
早产是全球公共卫生危机，导致新生儿和孕产妇大量死亡。然而，关于特发性自发性早产的分子机制知之甚少，我们也很少有足够的诊断标志物来评估胎盘的发育和功能。先前的胎盘病理学研究和我们的转录组学研究表明，胎盘成熟度在特发性自发性早产中起作用。众所周知，胎盘DNA甲基化在妊娠期间发生变化。我们假设，如果我们的样本中存在胎盘过度成熟，我们将观察到一种独特的特发性自发性早产DNA甲基化谱，可能会驱动我们之前在胎盘样本中发现的基因表达差异。我们的研究结果表明，特发性自发性早产DNA甲基化模式模仿足月出生甲基化模式，表明早产。只有7个显著的甲基化区域符合特发性自发性早产特异性（相对于对照组），表明特发性自发性早产和足月分娩样本之间的DNA甲基化异常相似。我们在胎龄匹配的对照组中发现了另外1718个显著甲基化区域，其中特发性自发性早产DNA甲基化模式模仿足月出生甲基化模式，再次表明特发性自发性早产和足月出生样本之间存在惊人的相似性。这些区域的通路分析揭示了WNT和Cadherin信号通路内基因的差异，这两者都是胎盘发育和成熟所必需的。综上所述，这些数据表明特发性自发性早产样本显示出比预期的高度成熟的甲基化特征，考虑到它们各自的胎龄，这可能会影响分娩时间。
Preterm birth is a global public health crisis which results in significant neonatal and maternal mortality. Yet little is known regarding the molecular mechanisms of idiopathic spontaneous preterm birth, and we have few diagnostic markers for adequate assessment of placental development and function. Previous studies of placental pathology and our transcriptomics studies suggest a role for placental maturity in idiopathic spontaneous preterm birth. It is known that placental DNA methylation changes over gestation. We hypothesized that if placental hypermaturity is present in our samples, we would observe a unique idiopathic spontaneous preterm birth DNA methylation profile potentially driving the gene expression differences we previously identified in our placental samples. Our results indicate the idiopathic spontaneous preterm birth DNA methylation pattern mimics the term birth methylation pattern suggesting hypermaturity. Only seven significant differentially methylated regions fitting the idiopathic spontaneous preterm birth specific (relative to the controls) profile were identified, indicating unusually high similarity in DNA methylation between idiopathic spontaneous preterm birth and term birth samples. We identified an additional 1,718 significantly methylated regions in our gestational age matched controls where the idiopathic spontaneous preterm birth DNA methylation pattern mimics the term birth methylation pattern, again indicating a striking level of similarity between the idiopathic spontaneous preterm birth and term birth samples. Pathway analysis of these regions revealed differences in genes within the WNT and Cadherin signaling pathways, both of which are essential in placental development and maturation. Taken together, these data demonstrate that the idiopathic spontaneous preterm birth samples display a hypermature methylation signature than expected given their respective gestational age which likely impacts birth timing.
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影响因子：
3.5
作者：
Beaumont RN;Warrington NM;Cavadino A;Tyrrell J;Nodzenski M;Horikoshi M;Geller F;Myhre R;Richmond RC;Paternoster L;Bradfield JP;Kreiner-Møller E;Huikari V;Metrustry S;Lunetta KL;Painter JN;Hottenga JJ;Allard C;Barton SJ;Espinosa A;Marsh JA;Potter C;Zhang G;Ang W;Berry DJ;Bouchard L;Das S;Early Growth Genetics (EGG) Consortium;Hakonarson H;Heikkinen J;Helgeland Ø;Hocher B;Hofman A;Inskip HM;Jones SE;Kogevinas M;Lind PA;Marullo L;Medland SE;Murray A;Murray JC;Njølstad PR;Nohr EA;Reichetzeder C;Ring SM;Ruth KS;Santa-Marina L;Scholtens DM;Sebert S;Sengpiel V;Tuke MA;Vaudel M;Weedon MN;Willemsen G;Wood AR;Yaghootkar H;Muglia LJ;Bartels M;Relton CL;Pennell CE;Chatzi L;Estivill X;Holloway JW;Boomsma DI;Montgomery GW;Murabito JM;Spector TD;Power C;Järvelin MR;Bisgaard H;Grant SFA;Sørensen TIA;Jaddoe VW;Jacobsson B;Melbye M;McCarthy MI;Hattersley AT;Hayes MG;Frayling TM;Hivert MF;Felix JF;Hyppönen E;Lowe WL Jr;Evans DM;Lawlor DA;Feenstra B;Freathy RM
通讯作者：
Freathy RM
影响因子：
5.8
作者：
Frendo, JL;Vidaud, M;Evain-Brion, D
通讯作者：
Evain-Brion, D
影响因子：
15.9
作者：
Hirota, Yasushi;Daikoku, Takiko;Dey, Sudhansu K.
通讯作者：
Dey, Sudhansu K.
影响因子：
4.3
作者：
Guo, Xiaohuan;Zhang, Yanfei;Han, Wenling
通讯作者：
Han, Wenling
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y