Structural Analysis of Bacillus subtilis Sigma Factors.

Structural Analysis of Bacillus subtilis Sigma Factors.
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DOI:
10.3390/microorganisms11041077
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发表时间:
2023-04-20
期刊:
影响因子:
4.5
通讯作者:
Isaacson RL
Isaacson RL
中科院分区:
生物学3区
文献类型:
--
作者:
Collins KM;Evans NJ;Torpey JH;Harris JM;Haynes BA;Camp AH;Isaacson RL

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细菌在其生命周期的不同阶段使用一系列sigma因子来调节基因表达。由于西格马因子的许多区域具有内在无序性,因此通过实验获得西格马因子的全长原子级结构一直具有挑战性。AlphaFold现在已经为大多数sigma因子提供了合理的全长模型。在这里,我们讨论了目前的理解的模式生物,枯草芽孢杆菌中的西格玛因子的结构和功能,并提出了一个区域的B的X射线晶体结构。枯草芽孢杆菌SigE,一种在孢子形成的发育过程中起关键作用的σ因子。
Bacteria use an array of sigma factors to regulate gene expression during different stages of their life cycles. Full-length, atomic-level structures of sigma factors have been challenging to obtain experimentally as a result of their many regions of intrinsic disorder. AlphaFold has now supplied plausible full-length models for most sigma factors. Here we discuss the current understanding of the structures and functions of sigma factors in the model organism, Bacillus subtilis, and present an X-ray crystal structure of a region of B. subtilis SigE, a sigma factor that plays a critical role in the developmental process of spore formation.
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