Th17 cells in primary Sjögren's syndrome negatively correlate with increased Roseburia and Coprococcus.
Th17 cells in primary Sjögren's syndrome negatively correlate with increased Roseburia and Coprococcus.
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原发性干燥综合征中的 Th17 细胞与罗斯伯利亚菌和粪球菌增加呈负相关
DOI：
10.3389/fimmu.2022.974648
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发表时间：
2022
影响因子：
7.3
通讯作者：
Li Y
中科院分区：
文献类型：
--
作者：
Xin X;Wang Q;Qing J;Song W;Gui Y;Li X;Li Y
肠道菌群失调与慢性全身性炎症和自身免疫密切相关，在原发性干燥综合征（pSS）的发病机制中起重要作用。pSS患者外周血T淋巴细胞亚群比例（Th 17/Treg）明显降低。我们的目的是确定pSS中肠道微生物群和Th 17/Treg之间的关联。在2018年12月1日至2019年8月31日期间入组了98例pSS患者和105例健康对照（NC）。收集pSS患者和健康对照者的基线信息和临床参数。进行16 S rRNA测序以表征肠道微生物组并鉴定患者和健康对照之间差异丰富的肠道微生物。最后，评估了肠道中特定细菌分类群的相对丰度与临床结果参数之间的关联。患有pSS的患者显示肠道微生物多样性和丰富度降低，产丁酸盐细菌（如罗斯拜瑞氏菌属和粪球菌属）的丰度降低，以及其他分类群（如直肠真杆菌和食菊罗斯拜瑞氏菌）的丰度增加。这些细菌富含与糖酵解和脂肪生成、能量、物质、半乳糖、戊糖代谢途径和葡萄糖醛酸相互转化相关的功能，而与肽聚糖生物合成、嘧啶代谢途径相关的功能减少。综合分析鉴定了肠道中pSS相关的特定细菌分类群，其中直肠真杆菌的丰度与Th 17/Treg呈负相关。此外，次级代谢物的生物合成、氨基酸的生物合成、肽聚糖的生物合成和嘧啶、半乳糖、戊糖、不同环境中的微生物代谢、乙醛酸和二羧酸代谢的途径与Treg或Th 17/Treg相关。原发性干燥综合征可导致患者肠道微生物多样性和肠道植物群丰富度降低。由微生物群诱导的Th 17和Treg细胞的比例是pSS的预测性表现，并解释了pSS的严重程度。
Dysbiosis of the gut microbiota is closely related to chronic systemic inflammation and autoimmunity, playing an essential role in the pathogenesis of primary Sjögren’s syndrome (pSS). Abnormalities in the proportions of blood T lymphocyte subtype, that is Th17/Treg, were detected in pSS patients. We aimed to determine the associations between gut microbiota and Th17/Treg in pSS. 98 pSS patients and 105 healthy controls (NC) were enrolled between Dec 1, 2018, and Aug 31, 2019. The baseline information and clinical parameters on pSS patients and healthy controls were collected. 16S rRNA sequencing was performed to characterize the gut microbiome and identify gut microbes that are differentially abundant between patients and healthy controls. Lastly, associations between relative abundances of specific bacterial taxa in the gut and clinical outcome parameters were evaluated. Patients with pSS show decreased gut microbial diversity and richness, decreased abundance of butyrate producing bacteria, such as Roseburia and Coprococcus, and increased abundance of other taxa, such as Eubacterium rectale and Roseburia inulinivorans. These bacteria are enriched with functions related to glycolytic and lipogenic, energy, substance, galactose, pentose metabolism pathways and glucuronate interconversions, decreased with functions related to peptidoglycan biosynthesis, pyrimidine metabolism pathways. An integrative analysis identified pSS-related specific bacterial taxa in the gut, for which the abundance of Eubacterium rectale is negatively correlated with Th17/Treg. Furthermore, the pathways of biosynthesis of secondary metabolites, biosynthesis of amino acids, peptidoglycan biosynthesis and pyrimidine, galactose, pentose, microbial metabolism in diverse environments, glyoxylate and dicarboxylate metabolism are associated with Treg or Th17/Treg. Primary Sjögren’s syndrome could lead to decreased gut microbial diversity and richness of intestinal flora in patients. The proportions of Th17 and Treg cells induced by microbiota were predictive pSS manifestations and accounted for the pSS severity.
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影响因子：
64.8
作者：
Bettelli, E;Carrier, YJ;Kuchroo, VK
通讯作者：
Kuchroo, VK
影响因子：
4.6
作者：
Bertorello, R;Cordone, MP;Cordone, G
通讯作者：
Cordone, G
影响因子：
6.4
作者：
Ellis, Jason S.;Wan, Xiaoxiao;Braley-Mullen, Helen
通讯作者：
Braley-Mullen, Helen
影响因子：
2.2
作者：
Kobayashi, Ichiro;Okura, Yuka;Ariga, Tadashi
通讯作者：
Ariga, Tadashi
影响因子：
6
作者：
Katsifis, Gikas E.;Rekka, Sofia;Wahl, Sharon M.
通讯作者：
Wahl, Sharon M.