Dietary conjugated linoleic acid supplementation alleviates high lipid-induced intestinal damage in grass carp (Ctenopharyngodon idella)
Dietary conjugated linoleic acid supplementation alleviates high lipid-induced intestinal damage in grass carp (Ctenopharyngodon idella)
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日粮补充共轭亚油酸可减轻草鱼（Ctenopharyngodon idella）高脂引起的肠道损伤
DOI：
10.46989/001c.23507
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发表时间：
2021-04
期刊：
影响因子：
--
通讯作者：
Denghang Yu
中科院分区：
文献类型：
--
作者：
Yang Yang;Hongsen Xu;Jiazhi Chang;Bing Hu;Jun Liu;Denghang Yu
高脂饮食可增加哺乳动物肠道通透性和炎症反应，共轭亚油酸（CLA）可减轻高脂饮食引起的症状。然而，有关高脂饲料对鱼类肠道的影响的研究有限，CLA对高脂饲料引起的鱼类肠道变化的影响尚不清楚。因此，本研究通过为期8周的饲养试验，探讨了CLA对高脂饲料诱导的草鱼肠道物理和免疫屏障损伤的潜在保护作用。3种等氮日粮，包括鱼粉对照日粮、添加3.5%豆油的高脂日粮（HL）和用2.5%共轭亚油酸（CLA）替代HL的高脂日粮（HL）。与对照组相比，HL组促炎基因（TNF-α、IL-8、IL-1β、NF-κB p65、IκBα、IKKα、IKKβ、IKKγ）的表达显著增加，抗炎细胞因子（IL-10）的表达显著下调。高脂饲料组鱼肠道紧密连接蛋白（ZO-1、闭合蛋白、claudin c和claudin B）的表达低于对照组。与HL组相比，CLA组促炎细胞因子基因表达降低，抗炎细胞因子基因表达升高。HE染色结果显示，高脂饲料诱导的鱼肠道损伤和饲料中CLA的损伤可减轻症状。因此，本研究显示共轭亚油酸可减轻高脂饲料所造成的草鱼肠道发炎及通透性的增加。
High lipid diet has been shown to increase the intestinal permeability and inflammatory properties in mammals, and the conjugated linoleic acid (CLA) could alleviate the symptoms caused by high lipid diet. However, the information about the impact of high lipid diet on the intestine of fish was limited, and the effect of CLA on intestinal changes induced by high lipid diet in fish is still unknown. Therefore, an 8-week feeding trial was conducted to investigate CLA's potential effects against high lipid diet-induced intestinal physical and immune barrier damage in grass carp (Ctenopharyngodon idella). Three isonitrogenous diets, including fish meal-control diet, control diet supplemented with 3.5% soybean oil as high lipid diet (HL), and HL replaced with a 2.5% CLA diet (CLA) formulated. Compared to the control group, HL significantly increased the expression of pro-inflammation genes (TNF-α, IL-8, IL-1β, NF-κB p65, IκBα, IKKα, IKKβ, and IKKγ) and down-regulated the gene expression of anti-inflammation cytokine (IL-10). The expression of intestinal tight junction proteins (ZO-1, occludin, claudin c, and claudin b) in fish fed with high lipid diets was lower than those fed with control diets. In the CLA group, the gene expression of pro-inflammation cytokines was decreased, and the expression level of the anti-inflammation cytokine was increased, compared with those in HL group. Moreover, feeding with high lipid diets induced fish intestine and dietary CLA damage could alleviate the symptom as shown by HE staining. In conclusion, this research revealed that CLA could mitigate high lipid diet-induced inflammation and permeability in the intestine of grass carp.
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影响因子：
3.4
作者：
Capaldo, Christopher T.;Nusrat, Asma
通讯作者：
Nusrat, Asma
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14.6
作者：
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DOI：
10.1016/j.bpg.2010.08.005
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2010-10-01
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作者：
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DOI：
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2016
期刊：
--
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作者：
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DOI：
10.1007/978-1-4471-4715-2_9
发表时间：
2021-10
期刊：
Clinical Cases in Hepatology
影响因子：
--
作者：
N. Bergasa
通讯作者：
N. Bergasa