Arabidopsis seed germination speed is controlled by SNL histone deacetylase-binding factor-mediated regulation of AUX1.
Arabidopsis seed germination speed is controlled by SNL histone deacetylase-binding factor-mediated regulation of AUX1.
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拟南芥种子发芽速度由 SNL 组蛋白脱乙酰酶结合因子介导的 AUX1 调节控制
DOI：
10.1038/ncomms13412
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发表时间：
2016-11-11
影响因子：
16.6
通讯作者：
Liu, Yongxiu
中科院分区：
文献类型：
--
作者：
Wang, Zhi;Chen, Fengying;Li, Xiaoying;Cao, Hong;Ding, Meng;Zhang, Cun;Zuo, Jinghong;Xu, Chaonan;Xu, Jimei;Deng, Xin;Xiang, Yong;Soppe, Wim J. J.;Liu, Yongxiu
已知组蛋白乙酰化会影响种子萌发的速度，但其分子调控基础仍不明确。在这里，我们报告的两个组蛋白脱乙酰酶结合因子，SWI-INDEPENDENT 3（SIN 3）-LIKE 1（SNL 1）和SNL 2的功能丧失，导致种子萌发过程中胚根的突起和生长加速。生长素抗性1（AUX 1）被确定为该过程中的关键因子，其增强SNL 1和SNL 2下游的发芽速度。AUX 1表达和组蛋白H3在赖氨酸9和18处的乙酰化受SNL 1和SNL 2调节。D-型细胞周期蛋白编码基因CYCD 1;1和CYCD 4;1在AUX 1过表达系和snl 1 snl 2双突变体中表现出增加的表达。因此，CYCD 4;1的敲除降低了AUX 1过表达株系和snl 1 snl 2的种子萌发速度，表明种子萌发过程中细胞周期对胚根伸出的重要性。总之，我们的工作确定AUX 1作为SNL 1和SNL 2介导的组蛋白乙酰化之间的联系，并在种子萌发过程中由CYCD 1;1和CYCD 4;1促进胚根生长。
Histone acetylation is known to affect the speed of seed germination, but the molecular regulatory basis of this remains ambiguous. Here we report that loss of function of two histone deacetylase-binding factors, SWI-INDEPENDENT3 (SIN3)-LIKE1 (SNL1) and SNL2, results in accelerated radicle protrusion and growth during seed germination. AUXIN RESISTANT 1 (AUX1) is identified as a key factor in this process, enhancing germination speed downstream of SNL1 and SNL2. AUX1 expression and histone H3 acetylation at lysines 9 and 18 is regulated by SNL1 and SNL2. The D-type cyclins encoding genes CYCD1;1 and CYCD4;1 display increased expression in AUX1 over-expression lines and the snl1snl2 double mutant. Accordingly, knockout of CYCD4;1 reduces seed germination speed of AUX1 over-expression lines and snl1snl2 suggesting the importance of cell cycling for radicle protrusion during seed germination. Together, our work identifies AUX1 as a link between histone acetylation mediated by SNL1 and SNL2, and radicle growth promoted by CYCD1;1 and CYCD4;1 during seed germination.
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影响因子：
64.8
作者：
Blilou, I;Xu, J;Scheres, B
通讯作者：
Scheres, B
影响因子：
4.5
作者：
Bouyer D;Roudier F;Heese M;Andersen ED;Gey D;Nowack MK;Goodrich J;Renou JP;Grini PE;Colot V;Schnittger A
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Paszkowski, J
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7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF