The predatory soil bacterium Lysobacter reprograms quorum sensing system to regulate antifungal antibiotic production in a cyclic-di-GMP-independent manner.
The predatory soil bacterium Lysobacter reprograms quorum sensing system to regulate antifungal antibiotic production in a cyclic-di-GMP-independent manner.
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捕食性土壤细菌溶杆菌重新编程群体感应系统，以不依赖环二 GMP 的方式调节抗真菌抗生素的生产
DOI：
10.1038/s42003-021-02660-7
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发表时间：
2021-09-24
影响因子：
5.9
通讯作者：
Liu F
中科院分区：
文献类型：
--
作者：
Li K;Xu G;Wang B;Wu G;Hou R;Liu F
土壤中的细菌常常携带各种毒素来对抗真核或原核生物。扩散信号因子（DSFs）代表了一组独特的群体感应（QS）化学物质，可调节不产生类抗生素分子的细菌的种间竞争。然而，DSF介导的QS系统调节抗生素生产的种间竞争的分子机制在土壤生防细菌中仍然是未知的。在这项研究中，我们发现来自溶杆菌的必要QS系统组分蛋白RpfG除了是环状二聚GMP（c-di-GMP）磷酸二酯酶（PDE）外，还调节抗真菌因子（热稳定抗真菌因子，HSAF）的生物合成，这似乎不依赖于酶活性。有趣的是，我们发现，RpfG相互作用与三个杂交双组分系统（HyTCS）蛋白，HtsH 1，HtsH 2和HtsH 3，调节溶杆菌HSAF生产。体外研究表明，这些蛋白质中的每一个都与RpfG相互作用，这降低了RpfG的PDE活性。最后，我们表明，这些蛋白质的细胞质比例依赖于它们的磷酸化活性和结合的启动子控制的基因参与HSAF的合成。这些发现揭示了一个以前未知的机制DSF信号在抗生素生产的土壤细菌。Li等人表明，来自溶杆菌的群体感应系统组分蛋白RpfG除了是环状二聚GMP（c-di-GMP）磷酸二酯酶之外，还调节抗真菌因子的生物合成。他们表明，RpfG通过与三种杂交双组分系统（HyTCS）蛋白的直接相互作用来调节HSAF的产生，从而为土壤细菌的抗真菌防御提供了见解。
Soil bacteria often harbour various toxins to against eukaryotic or prokaryotic. Diffusible signal factors (DSFs) represent a unique group of quorum sensing (QS) chemicals that modulate interspecies competition in bacteria that do not produce antibiotic-like molecules. However, the molecular mechanism by which DSF-mediated QS systems regulate antibiotic production for interspecies competition remains largely unknown in soil biocontrol bacteria. In this study, we find that the necessary QS system component protein RpfG from Lysobacter, in addition to being a cyclic dimeric GMP (c-di-GMP) phosphodiesterase (PDE), regulates the biosynthesis of an antifungal factor (heat-stable antifungal factor, HSAF), which does not appear to depend on the enzymatic activity. Interestingly, we show that RpfG interacts with three hybrid two-component system (HyTCS) proteins, HtsH1, HtsH2, and HtsH3, to regulate HSAF production in Lysobacter. In vitro studies show that each of these proteins interacted with RpfG, which reduced the PDE activity of RpfG. Finally, we show that the cytoplasmic proportions of these proteins depended on their phosphorylation activity and binding to the promoter controlling the genes implicated in HSAF synthesis. These findings reveal a previously uncharacterized mechanism of DSF signalling in antibiotic production in soil bacteria. Li et al shows that the quorum sensing system component protein RpfG from Lysobacter, in addition to being a cyclic dimeric GMP (c-di-GMP) phosphodiesterase, also regulates the biosynthesis of an antifungal factor. They show that RpfG regulates the production of HSAF through a direct interaction with three hybrid two component system (HyTCS) proteins, providing insights into the antifungal defence in soil bacteria.
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