Dynamics and stoichiometry of a regulated enhancer-binding protein in live Escherichia coli cells.
Dynamics and stoichiometry of a regulated enhancer-binding protein in live Escherichia coli cells.
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DOI：
10.1038/ncomms2997
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发表时间：
2013
影响因子：
16.6
通讯作者：
中科院分区：
文献类型：
--
作者：
细菌增强子依赖的转录系统支持主要的适应性反应，并提供了一个单一的基因控制模式，类似于复杂的真核系统。在这里，我们报告了新的机制的见解控制单膜应力响应细菌增强子依赖性系统。使用毫秒单分子荧光显微镜的活细胞，我们确定的本地化，二维扩散动力学和化学计量的复合物的细菌增强子结合ATP酶PspF在其作用在启动子调节内膜相互作用负控制器PspA。我们建立了一个稳定的抑制性PspF-PspA复合物位于类核中，通过PspA与内膜短暂通讯。PspF作为一个六聚体稳定地结合只有一个两个PSP启动子的时间，这表明PSP启动子将火异步和合作的相互作用PspF与基础的转录复合物的影响动力学的PspF六聚体-DNA复合物和调节的PSP启动子。 细胞适应性反应需要时间和空间的关键调控蛋白复合物的控制。Mehta等描述了在大肠杆菌中介导膜应激反应的转录激活因子的动态相互作用。大肠杆菌及其负调控因子、细胞膜和转录机制。
Bacterial enhancer-dependent transcription systems support major adaptive responses and offer a singular paradigm in gene control analogous to complex eukaryotic systems. Here we report new mechanistic insights into the control of one-membrane stress-responsive bacterial enhancer-dependent system. Using millisecond single-molecule fluorescence microscopy of live cells we determine the localizations, two-dimensional diffusion dynamics and stoichiometries of complexes of the bacterial enhancer-binding ATPase PspF during its action at promoters as regulated by inner membrane interacting negative controller PspA. We establish that a stable repressive PspF–PspA complex is located in the nucleoid, transiently communicating with the inner membrane via PspA. The PspF as a hexamer stably binds only one of the two psp promoters at a time, suggesting that psp promoters will fire asynchronously and cooperative interactions of PspF with the basal transcription complex influence dynamics of the PspF hexamer–DNA complex and regulation of the psp promoters. Cellular adaptive responses require temporal and spatial control of key regulatory protein complexes. Mehta et al. describe the dynamic interaction of a transcriptional activator mediating membrane stress response in E. coli with its negative regulator, the cell membrane and the transcription machinery.
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影响因子：
56.9
作者：
Loehlin, David W.;Werren, John H.
通讯作者：
Werren, John H.
影响因子：
5.6
作者：
Joly N;Burrows PC;Engl C;Jovanovic G;Buck M
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影响因子：
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作者：
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Zhang, Xiaodong
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通讯作者：
Leake MC
影响因子：
3.2
作者：
Jovanovic, G;Dworkin, J;Model, P
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Model, P