Identification of NeuN immunopositive cells in the adult mouse subventricular zone
Identification of NeuN immunopositive cells in the adult mouse subventricular zone
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成年小鼠脑室下区 NeuN 免疫阳性细胞的鉴定
DOI：
10.1002/cne.24463
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发表时间：
2018
影响因子：
2.5
通讯作者：
Mori Tetsuji
中科院分区：
文献类型：
--
作者：
Saito Kengo;Koike Taro;Kawashima Fumiaki;Kurata Hirofumi;Shibuya Taku;Satoh Takemasa;Hata Yoshio;Yamada Hisao;Mori Tetsuji
在成年啮齿动物的脑室下区(SVZ)中，存在神经干细胞(NSCs)，而专门的神经源性生态位是维持其干性的关键。到目前为止，许多构成神经源性生态位的细胞和非细胞因子以及识别A型细胞亚群的标记已经得到证实。特别是，神经递质调节成人神经发生，对SVZ中的成熟神经元只进行了部分分析。此外，A型细胞是神经干细胞的后代，具有高度的异质性，仍需要更多的分子标记来鉴定它们。在本研究中，我们系统地对成年小鼠SVZ内免疫阳性(+)细胞进行了分类，NeuN通常被用作成熟和未成熟有丝分裂后神经元的标志。这些SVZ-Neun+细胞(SVZ-NS)主要分为两类。一种为成熟SVZ-NS(M-SVZ-NS)。M-SVZ-NS的神经化学特性与纹状体神经元相似，但出生日期和形态不同。M-SVZ-NS在胚胎和出生后早期产生，呈双极峰，突起沿侧脑室壁延伸。第二种类型为小SVZ-NS(S-SVZ-NS)，具有A型细胞特征。它们不仅表达A型细胞的标志物，而且还能从SVZ向嗅球增殖和迁移。此外，S-SVZ-NS根据其空间位置和谷氨酸脱羧酶67的表达可分为两类。我们的数据表明，M-SVZ-NS是神经源性生态位的一个新成分，S-SVZ-NS是新发现的A型细胞亚群。
In the adult rodent subventricular zone (SVZ), there are neural stem cells (NSCs) and the specialized neurogenic niche is critical to maintain their stemness. To date, many cellular and noncellular factors that compose the neurogenic niche and markers to identify subpopulations of Type A cells have been confirmed. In particular, neurotransmitters regulate adult neurogenesis and mature neurons in the SVZ have been only partially analyzed. Moreover, Type A cells, descendants of NSCs, are highly heterogeneous and more molecular markers are still needed to identify them. In the present study, we systematically classified NeuN, commonly used as a marker of mature and immature post‐mitotic neurons, immunopositive (+) cells within the adult mouse SVZ. These SVZ‐NeuN+ cells (SVZ‐Ns) were mainly classified into two types. One was mature SVZ‐Ns (M‐SVZ‐Ns). Neurochemical properties of M‐SVZ‐Ns were similar to those of striatal neurons, but their birth date and morphology were different. M‐SVZ‐Ns were generated during embryonic and early postnatal stages with bipolar peaks and extended their processes along the wall of the lateral ventricle. The second type was small SVZ‐Ns (S‐SVZ‐Ns) with features of Type A cells. They expressed not only markers of Type A cells, but also proliferated and migrated from the SVZ to the olfactory bulb. Furthermore, S‐SVZ‐Ns could be classified into two types by their spatial locations and glutamic acid decarboxylase 67 expression. Our data indicate that M‐SVZ‐Ns are a new component of the neurogenic niche and S‐SVZ‐Ns are newly identified subpopulations of Type A cells.
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影响因子：
4.7
作者：
Kim Y;Wang WZ;Comte I;Pastrana E;Tran PB;Brown J;Miller RJ;Doetsch F;Molnár Z;Szele FG
通讯作者：
Szele FG
影响因子：
23.9
作者：
Tong, Cheuk Ka;Chen, Jiadong;Cebrian-Silla, Arantxa;Mirzadeh, Zaman;Obernier, Kirsten;Guinto, Cristina D.;Tecott, Laurence H.;Manuel Garcia-Verdugo, Jose;Kriegstein, Arnold;Alvarez-Buylla, Arturo
通讯作者：
Alvarez-Buylla, Arturo
大鼠新纹状体的神经发生
DOI：
10.1016/0736-5748(84)90008-x
发表时间：
1984
影响因子：
1.8
作者：
S. A. Bayer
通讯作者：
S. A. Bayer
影响因子：
56.9
作者：
Sawamoto, K;Wichterle, H;Alvarez-Buylla, A
通讯作者：
Alvarez-Buylla, A
DOI：
10.1002/neu.480250206
发表时间：
1994-02-01
期刊：
JOURNAL OF NEUROBIOLOGY
影响因子：
--
作者：
MARUSICH, MF;FURNEAUX, HM;WESTON, JA
通讯作者：
WESTON, JA