Alternative Splicing of Testis-Specific Lactate Dehydrogenase C Gene in Mammals and Pigeon
Alternative Splicing of Testis-Specific Lactate Dehydrogenase C Gene in Mammals and Pigeon
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哺乳动物和鸽子睾丸特异性乳酸脱氢酶 C 基因的选择性剪接
DOI：
10.1080/10495398.2011.652325
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发表时间：
2012-04
影响因子：
3.7
通讯作者：
郑玉才
中科院分区：
文献类型：
--
作者：
黄林;林亚秋;刘薇;徐亚欧;郑玉才
本研究的目的是证实乳酸脱氢酶c（ldhc）基因选择性剪接在哺乳动物中的广泛存在。采用RT-PCR方法从猪、狗、兔、猫、大鼠、小鼠和鸽子的睾丸中扩增ldhc的cDNA。在7个物种中观察到2 ~ 6种ldhc剪接变异体，这是由于成熟mRNA中一个或多个外显子缺失或内含子部分序列插入的结果。缺失的外显子主要发生在外显子5、4、6和3。仅在狗和大鼠中观察到内含子的部分序列的插入，这导致插入的内含子序列中的异常终止密码子。外显子的缺失还导致阅读移码和在一些变体中终止密码子的形成。牦牛睾丸中ldha和ldhb基因未发现选择性剪接。天然聚丙烯酰胺凝胶电泳和Western印迹分析显示，没有明显的LDH-C4活性来自表达ldhc的变体。我们的研究结果证明了ldhc基因选择性剪接在哺乳动物中广泛而独特的存在。
The objective of the present study was to confirm the widespread existence of alternative splicing of lactate dehydrogenase c (ldhc) gene in mammals. RT-PCR was employed to amplify cDNAs of ldhc from testes of mammals including pig, dog, rabbit, cat, rat, and mouse, as well as pigeon. Two to six kinds of splice variants of ldhc were observed in the seven species as a result of deletion of one or more exons or insertion of partial sequence of an intron in the mature mRNA. The deleted exons occur mostly in exons 5, 4, 6, and 3. The insertion of a partial sequence of introns, which resulted in an abnormal stop codon in the inserted intron sequence, was observed only in dog and rat. The deletion of exons also resulted in a reading frame shift and formation of a stop codon in some variants. No alternative splicing was observed for ldha and ldhb genes in testis of yak. Native polyacrylamide gel electrophoresis and Western blot analysis revealed no obvious LDH-C4 activity derived from expressed ldhc variants. Our results demonstrated the widespread and unique existence of alternative splicing of ldhc genes in mammals.
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影响因子：
64.8
作者：
通讯作者：
--
影响因子：
3.6
作者：
Kroft, TL;Jethanandani, P;Goldberg, E
通讯作者：
Goldberg, E
影响因子：
4.1
作者：
Thangaraju, Muthusamy;Carswell, Kristina N.;Ganapathy, Vadivel
通讯作者：
Ganapathy, Vadivel
DOI：
10.1002/jez.1401560202
发表时间：
1964-07
期刊：
The Journal of experimental zoology
影响因子：
--
作者：
A. Blanco;W. Zinkham;L. Kupchyk
通讯作者：
A. Blanco;W. Zinkham;L. Kupchyk
影响因子：
--
作者：
Coonrod, Scott;Vitale, Alejandra;Goldberg, Erwin
通讯作者：
Goldberg, Erwin