DELLA activity is required for successful pollen development in the Columbia ecotype of Arabidopsis.
DELLA activity is required for successful pollen development in the Columbia ecotype of Arabidopsis.
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DOI：
10.1111/nph.12571
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发表时间：
2014-02
期刊：
影响因子：
--
通讯作者：
Thomas SG
中科院分区：
文献类型：
--
作者：
Plackett ARG;Ferguson AC;Powers SJ;Wanchoo-Kohli A;Phillips AL;Wilson ZA;Hedden P;Thomas SG
过量的赤霉素(GA)信号通过Della蛋白介导，对植物的育性产生负面影响。在单子叶水稻中，Della活性的丧失会导致完全雄性不育，但在双子叶模式拟南芥(Arabitopsis Thaliana)生态型Landsberg erecta(LER)中，Della活性的丧失则不会导致雄性不育，这导致了Della活性对拟南芥花粉发育不是必需的假设。在哥伦比亚(COL-0)生态型中发现了一种新的della育性表型，这需要重新评估从LER得出的一般结论。在化学和遗传GA过量的条件下，比较了Col-0和Ler生态型之间的育性表型，包括两个生态型中缺乏Della类似物抑制子GA1-3(RGA)和GA不敏感(GAI)的突变体。在GA处理下，LER表现出比Col-0更轻的育性表型。与相应的LER表型相比，Col-0 RGA gai突变体完全雄性不育，这是由花粉发育中的减数分裂后缺陷引起的，这些缺陷是通过将della重新导入绒毡层或发育中的花粉而挽救的。我们得出结论：Della活性对拟南芥花粉发育是必不可少的。COL-0和LER育性反应的差异可能是由于下游信号通路的差异或della表达的改变造成的。
Excessive gibberellin (GA) signalling, mediated through the DELLA proteins, has a negative impact on plant fertility. Loss of DELLA activity in the monocot rice (Oryza sativa) causes complete male sterility, but not in the dicot model Arabidopsis (Arabidopsis thaliana) ecotype Landsberg erecta (Ler), in which DELLA function has been studied most extensively, leading to the assumption that DELLA activity is not essential for Arabidopsis pollen development. A novel DELLA fertility phenotype was identified in the Columbia (Col-0) ecotype that necessitates re-evaluation of the general conclusions drawn from Ler. Fertility phenotypes were compared between the Col-0 and Ler ecotypes under conditions of chemical and genetic GA overdose, including mutants in both ecotypes lacking the DELLA paralogues REPRESSOR OF ga1-3 (RGA) and GA INSENSITIVE (GAI). Ler displays a less severe fertility phenotype than Col-0 under GA treatment. Col-0 rga gai mutants, in contrast with the equivalent Ler phenotype, were entirely male sterile, caused by post-meiotic defects in pollen development, which were rescued by the reintroduction of DELLA into either the tapetum or developing pollen. We conclude that DELLA activity is essential for Arabidopsis pollen development. Differences between the fertility responses of Col-0 and Ler might be caused by differences in downstream signalling pathways or altered DELLA expression.
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影响因子：
11.6
作者：
Griffiths, Jayne;Murase, Kohji;Thomas, Stephen G.
通讯作者：
Thomas, Stephen G.
影响因子：
64.8
作者：
Gan X;Stegle O;Behr J;Steffen JG;Drewe P;Hildebrand KL;Lyngsoe R;Schultheiss SJ;Osborne EJ;Sreedharan VT;Kahles A;Bohnert R;Jean G;Derwent P;Kersey P;Belfield EJ;Harberd NP;Kemen E;Toomajian C;Kover PX;Clark RM;Rätsch G;Mott R
通讯作者：
Mott R
影响因子：
56.9
作者：
Alonso, JM;Stepanova, AN;Ecker, JR
通讯作者：
Ecker, JR
影响因子：
4.9
作者：
Hirano K;Aya K;Hobo T;Sakakibara H;Kojima M;Shim RA;Hasegawa Y;Ueguchi-Tanaka M;Matsuoka M
通讯作者：
Matsuoka M
DOI：
10.1073/pnas.0608936104
发表时间：
2007-03-06
影响因子：
11.1
作者：
Francis, Kirk E.;Lam, Sandy Y.;Copenhaver, Gregory P.
通讯作者：
Copenhaver, Gregory P.