Proteomic analysis of salt-stressed tomato (Solanum lycopersicum) seedlings: effect of genotype and exogenous application of glycinebetaine.
Proteomic analysis of salt-stressed tomato (Solanum lycopersicum) seedlings: effect of genotype and exogenous application of glycinebetaine.
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盐胁迫番茄（Solanum lycopersicum）幼苗的蛋白质组分析：基因型和外源施用甘氨酸甜菜碱的影响。
DOI：
10.1093/jxb/erp075
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发表时间：
2009
影响因子：
6.9
通讯作者：
Heuer B
中科院分区：
文献类型：
--
作者：
Chen S;Gollop N;Heuer B
以7 d龄番茄Solanum lycopersicum（L.）磨坊品种Patio和'F144'，使用蛋白质组学方法。从非盐对照和盐胁迫幼苗的胚根和下胚轴中提取总蛋白，并通过双向凝胶电泳进行分离。液相色谱-电喷雾串联质谱（LC-ESI-MS/MS）鉴定了23个盐胁迫反应蛋白，分为6类。研究了外源甜菜碱（GB）对盐胁迫诱导的Patio和F144番茄幼苗生长抑制及蛋白质谱的影响。结果表明，与盐胁迫幼苗相比，GB可使Patio中的6种蛋白和F144中的2种蛋白的表达丰度增加2倍以上，从而缓解盐胁迫对番茄生长的抑制。此外，基于计算生物信息学的相互作用分析揭示了主要的调控网络：光系统II（PSII），Rubisco和超氧化物歧化酶（SOD）。结果表明，外源亲和性溶质如GB的应用可能会提高番茄的耐盐性。此外，对盐胁迫下番茄差异表达蛋白质的定量和定性分析是进一步阐明番茄抗盐机制的重要一步。
An investigation aimed at a better understanding of the molecular adaptation mechanisms of salt stress was carried out in 7-d-old tomato Solanum lycopersicum (L.) Mill cultivars Patio and ‘F144’, using a proteomic approach. Total proteins were extracted from radicles and hypocotyls collected from both non-saline control and salt-stressed seedlings, and separated by two-dimensional gel electrophoresis. Liqud chromatography-electron spray ionization tandem mass spectrometry (LC-ESI-MS/MS) identified 23 salt stress response proteins, classified into six functional categories. The effect of exogenously applied glycinebetaine (GB) on the salt stress-induced inhibition of growth in tomato seedlings of cultivars Patio and ‘F144’ and on the protein profile was investigated. It was found that GB could alleviate the inhibition of tomato growth induced by salt stress through changing the expression abundance of six proteins in Patio and two proteins in ‘F144’ more than twice compared with salt-stressed seedlings. Furthermore, the interaction analysis based on computational bioinformatics reveals major regulating networks: photosystem II (PSII), Rubisco, and superoxide dismutase (SOD). The results suggest that it is likely that improvement of salt tolerance in tomato might be achieved through the application of exogenous compatible solutes, such as GB. Moreover, quantitative and qualitative analysis of the differentially expressed proteins of tomato under salt stress is an important step towards further elucidation of mechanisms of salt stress resistance.
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影响因子：
4.8
作者：
Bugos, RC;Hieber, AD;Yamamoto, HY
通讯作者：
Yamamoto, HY
影响因子：
7.3
作者：
KISHITANI, S;WATANABE, K;TAKABE, T
通讯作者：
TAKABE, T
影响因子：
5.2
作者：
Makela, P;PeltonenSainio, P;Somersalo, S
通讯作者：
Somersalo, S
影响因子：
3.5
作者：
MAMEDOV, MD;HAYASHI, H;MURATA, N
通讯作者：
MURATA, N
影响因子：
1.5
作者：
Chen, SB;Glazer, I;Wilson, MJ
通讯作者：
Wilson, MJ