Skeletal myogenic progenitors originating from embryonic dorsal aorta coexpress endothelial and myogenic markers and contribute to postnatal muscle growth and regeneration.
Skeletal myogenic progenitors originating from embryonic dorsal aorta coexpress endothelial and myogenic markers and contribute to postnatal muscle growth and regeneration.
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DOI：
10.1083/jcb.147.4.869
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发表时间：
1999-11-15
影响因子：
7.8
通讯作者：
Cossu, G
中科院分区：
文献类型：
--
作者：
De Angelis, L;Berghella, L;Coletta, M;Lattanzi, L;Zanchi, M;Cusella-De Angelis, MG;Ponzetto, C;Cossu, G
脊椎动物的骨骼肌来源于体节，即轴旁中胚层的上皮结构，然而许多不相关的报道描述了偶尔会从非体节来源的组织中出现成肌细胞，这表明要么是转分化，要么是多能祖细胞的持续存在。在此，我们表明可克隆的骨骼肌成肌细胞存在于小鼠胚胎的胚胎背主动脉中。这一发现基于对不同发育阶段的不同组织原基的详细克隆分析。在体外，这些成肌细胞显示出与源自成年骨骼肌的卫星细胞相同的形态，并表达多种成肌和内皮标记物。令人惊讶的是，成年卫星细胞也表达后者。此外，从缺乏附肢肌肉但具有正常血管系统的突变型c - Met−/−胚胎的肢体中克隆成肌细胞是可能的。在移植后，主动脉来源的成肌细胞参与出生后肌肉的生长和再生，并与驻留的卫星细胞融合。
血管系统产生骨骼肌细胞的潜能可能解释了非体节骨骼肌生成的观察结果，并提出了卫星细胞的一个子集可能来源于血管系统的可能性。
Skeletal muscle in vertebrates is derived from somites, epithelial structures of the paraxial mesoderm, yet many unrelated reports describe the occasional appearance of myogenic cells from tissues of nonsomite origin, suggesting either transdifferentiation or the persistence of a multipotent progenitor. Here, we show that clonable skeletal myogenic cells are present in the embryonic dorsal aorta of mouse embryos. This finding is based on a detailed clonal analysis of different tissue anlagen at various developmental stages. In vitro, these myogenic cells show the same morphology as satellite cells derived from adult skeletal muscle, and express a number of myogenic and endothelial markers. Surprisingly, the latter are also expressed by adult satellite cells. Furthermore, it is possible to clone myogenic cells from limbs of mutant c-Met−/− embryos, which lack appendicular muscles, but have a normal vascular system. Upon transplantation, aorta-derived myogenic cells participate in postnatal muscle growth and regeneration, and fuse with resident satellite cells. The potential of the vascular system to generate skeletal muscle cells may explain observations of nonsomite skeletal myogenesis and raises the possibility that a subset of satellite cells may derive from the vascular system.
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影响因子：
56.9
作者：
Asahara, T;Murohara, T;Isner, JM
通讯作者：
Isner, JM
影响因子：
2.7
作者：
Cornelison, DDW;Wold, BJ
通讯作者：
Wold, BJ
DOI：
10.1083/jcb.106.6.2139
发表时间：
1988-06
期刊：
The Journal of cell biology
影响因子：
--
作者：
Grigoriadis AE;Heersche JN;Aubin JE
通讯作者：
Aubin JE
影响因子：
56.9
作者：
Ferrari, G;Cusella-De Angelis, G;Mavilio, F
通讯作者：
Mavilio, F
影响因子：
2.7
作者：
Garry, DJ;Yang, QA;Williams, RS
通讯作者：
Williams, RS