Comprehensive behavioral analysis of cluster of differentiation 47 knockout mice.
Comprehensive behavioral analysis of cluster of differentiation 47 knockout mice.
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DOI：
10.1371/journal.pone.0089584
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Miyakawa T
中科院分区：
文献类型：
--
作者：
Koshimizu H;Takao K;Matozaki T;Ohnishi H;Miyakawa T
分化簇47（CD47）是免疫球蛋白超家族的成员，其作为信号调节蛋白α（SIRPα）的胞外区的配体起作用，SIRP α是在脑中大量表达的蛋白质。包括我们在内的研究表明，CD47和SIRPα在中枢神经系统（CNS）中发挥着多种功能，如调节突触传递和神经细胞存活。我们曾报道CD47通过调节SIRPα的酪氨酸磷酸化参与了强迫游泳实验中小鼠抑郁样行为的调节。然而，CD47的其他潜在行为功能在很大程度上仍然未知。在这项研究中，为了进一步研究CD47在CNS中的功能作用，对CD47敲除（KO）小鼠及其野生型同窝仔进行了一系列行为测试。CD47基因敲除小鼠表现出前脉冲抑制降低，而惊吓反应在基因型之间没有差异。突变体表现出轻微的，但显着降低社交性和社会新奇的偏好在克劳利的三室社会方法测试，而在社会互动测试中，实验和刺激小鼠有直接接触，在一个自由移动的设置在一个新的环境或家庭笼，基因型之间没有显着差异。虽然以前的研究表明，CD47调节啮齿类动物的抑制性回避试验中的恐惧记忆，我们的CD47 KO小鼠表现出正常的恐惧和空间记忆的恐惧条件反射和巴恩斯迷宫测试，分别。这些发现表明，CD47可能参与小鼠感觉运动门控和社会行为的调节。
Cluster of differentiation 47 (CD47) is a member of the immunoglobulin superfamily which functions as a ligand for the extracellular region of signal regulatory protein α (SIRPα), a protein which is abundantly expressed in the brain. Previous studies, including ours, have demonstrated that both CD47 and SIRPα fulfill various functions in the central nervous system (CNS), such as the modulation of synaptic transmission and neuronal cell survival. We previously reported that CD47 is involved in the regulation of depression-like behavior of mice in the forced swim test through its modulation of tyrosine phosphorylation of SIRPα. However, other potential behavioral functions of CD47 remain largely unknown. In this study, in an effort to further investigate functional roles of CD47 in the CNS, CD47 knockout (KO) mice and their wild-type littermates were subjected to a battery of behavioral tests. CD47 KO mice displayed decreased prepulse inhibition, while the startle response did not differ between genotypes. The mutants exhibited slightly but significantly decreased sociability and social novelty preference in Crawley’s three-chamber social approach test, whereas in social interaction tests in which experimental and stimulus mice have direct contact with each other in a freely moving setting in a novel environment or home cage, there were no significant differences between the genotypes. While previous studies suggested that CD47 regulates fear memory in the inhibitory avoidance test in rodents, our CD47 KO mice exhibited normal fear and spatial memory in the fear conditioning and the Barnes maze tests, respectively. These findings suggest that CD47 is potentially involved in the regulation of sensorimotor gating and social behavior in mice.
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影响因子：
2
作者：
Chang, HP;Lindberg, FP;Lee, EHY
通讯作者：
Lee, EHY
影响因子：
4.8
作者：
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通讯作者：
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DOI：
10.3791/1088
发表时间：
2008-12-22
期刊：
Journal of visualized experiments : JoVE
影响因子：
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作者：
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通讯作者：
Miyakawa, Tsuyoshi
影响因子：
2.7
作者：
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通讯作者：
Wrenn, Craige C.
影响因子：
4.7
作者：
Araki, T;Yamada, M;Hatanaka, H
通讯作者：
Hatanaka, H