Loss of the SPHF homologue Slr1768 leads to a catastrophic failure in the maintenance of thylakoid membranes in Synechocystis sp. PCC 6803.
Loss of the SPHF homologue Slr1768 leads to a catastrophic failure in the maintenance of thylakoid membranes in Synechocystis sp. PCC 6803.
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DOI：
10.1371/journal.pone.0019625
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Spence EM
中科院分区：
文献类型：
--
作者：
Bryan SJ;Burroughs NJ;Evered C;Sacharz J;Nenninger A;Mullineaux CW;Spence EM
在蓝细菌中，光系统被定位于并维持在称为类囊体的特殊膜中。驱动类囊体膜生物发生的机制仍然是一个悬而未决的问题，目前只确定了两个潜在的生物发生因子，Vipp 1和Alb 3。我们使用pGEM T-easy载体和REDIRECT产生slr 1768敲除。通过比较Δ slr 1768突变体与野生型的生长和色素含量（叶绿素a荧光），我们发现Δ slr 1768具有条件表型;特别是在强光条件下（130 µmol m−2 s−1），类囊体生物合成被破坏，导致细胞死亡。类囊体显示出相当大的破坏，结构和密度都损失，而叶绿素a密度随着类囊体的损失而降低，尽管光合效率不受影响。在弱光（30 µmol m−2 s−1）下，表型显著降低，生长速率与野生型相似，只有低频率的细胞具有明显的类囊体破坏。这是第一个例子的基因，影响维持类囊体膜特别是在强光下，并显示依赖于光强度的表型。我们的研究结果表明，SLR 1768具有主导作用的驯化，连接光损伤与类囊体的维护。
In cyanobacteria the photosystems are localised to, and maintained in, specialist membranes called the thylakoids. The mechanism driving the biogenesis of the thylakoid membranes is still an open question, with only two potential biogenesis factors, Vipp1 and Alb3 currently identified. We generated a slr1768 knockout using the pGEM T-easy vector and REDIRECT. By comparing growth and pigment content (chlorophyll a fluoresence) of the Δslr1768 mutant with the wild-type, we found that Δslr1768 has a conditional phenotype; specifically under high light conditions (130 µmol m−2 s−1) thylakoid biogenesis is disrupted leading to cell death on a scale of days. The thylakoids show considerable disruption, with loss of both structure and density, while chlorophyll a density decreases with the loss of thylakoids, although photosynthetic efficiency is unaffected. Under low light (30 µmol m−2 s−1) the phenotype is significantly reduced, with a growth rate similar to the wild-type and only a low frequency of cells with evident thylakoid disruption. This is the first example of a gene that affects the maintenance of the thylakoid membranes specifically under high light, and which displays a phenotype dependent on light intensity. Our results demonstrate that Slr1768 has a leading role in acclimatisation, linking light damage with maintenance of the thylakoids.
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影响因子：
6.5
作者：
Aseeva, Elena;Ossenbuehl, Friederich;Vothknecht, Ute C.
通讯作者：
Vothknecht, Ute C.
影响因子：
7.4
作者：
Fuhrmann, Eva;Gathmann, Sven;Schneider, Dirk
通讯作者：
Schneider, Dirk
DOI：
10.1071/pp99027
发表时间：
1999-01-01
期刊：
AUSTRALIAN JOURNAL OF PLANT PHYSIOLOGY
影响因子：
--
作者：
Mullineaux, CW
通讯作者：
Mullineaux, CW
DOI：
10.1073/pnas.0337542100
发表时间：
2003-02-18
影响因子：
11.1
作者：
Gust, B;Challis, GL;Chater, KF
通讯作者：
Chater, KF
影响因子：
4.8
作者：
Gerdes, Lars;Bals, Thomas;Schuenemann, Danja
通讯作者：
Schuenemann, Danja