Variation in dehydration tolerance, ABA sensitivity and related gene expression patterns in D-genome progenitor and synthetic hexaploid wheat lines.
Variation in dehydration tolerance, ABA sensitivity and related gene expression patterns in D-genome progenitor and synthetic hexaploid wheat lines.
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DOI：
10.3390/ijms10062733
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发表时间：
2009-06-18
影响因子：
5.6
通讯作者：
Takumi S
中科院分区：
文献类型：
--
作者：
Kurahashi Y;Terashima A;Takumi S
野生小麦。具有广泛的自然变异，可用于普通小麦的育种。干旱胁迫耐受性与ABA敏感性密切相关。以四倍体小麦品种兰登为材料，与17个品种杂交获得17个合成的六倍体小麦品系。以黄豆属（tauschii）为材料，比较分析其抗旱性和ABA敏感性的自然变异。Ae。陶氏性状表现出广泛的自然变异，性状间的相关性较弱。干旱敏感品种Ae。黄家鱼对ABA的敏感性明显降低。在二倍体基因组水平上观察到的d基因组变异并不一定反映在合成小麦中。然而，合成小麦来源于亲本Ae。耐旱性高的陶氏品种对干旱胁迫的耐受性显著高于干旱敏感品种。此外，高耐旱性合成小麦对ABA的敏感性显著高于对干旱敏感的合成品系。因此，在六倍体遗传背景下，ABA敏感性与抗旱性弱相关。为了研究耐胁迫系和敏感系基因表达模式的差异，比较了两种Cor/Lea和三种转录因子基因转录本的水平。耐蚀性较强的Ae。在胁迫条件下，黄家菜比敏感植株积累了更多的检测基因转录本。在干旱和ABA处理的亲本系中，合成小麦的表达模式似乎是加性的。然而，合成小麦中转录因子基因的转录水平并不一定符合基于亲本系表达的假设水平。异源多倍体化改变了合成小麦逆境响应基因的表达水平。
The wild wheat Aegilops tauschii Coss. has extensive natural variation available for breeding of common wheat. Drought stress tolerance is closely related to abscisic acid (ABA) sensitivity. In this study, 17 synthetic hexaploid wheat lines, produced by crossing the tetraploid wheat cultivar Langdon with 17 accessions of Ae. tauschii, were used for comparative analysis of natural variation in drought tolerance and ABA sensitivity. Ae. tauschii showed wide natural variation, with weak association between the traits. Drought-sensitive accessions of Ae. tauschii exhibited significantly less ABA sensitivity. D-genome variations observed at the diploid genome level were not necessarily reflected in synthetic wheats. However, synthetic wheats derived from the parental Ae. tauschii accessions with high drought tolerance were significantly more tolerant to drought stress than those from drought-sensitive accessions. Moreover, synthetic wheats with high drought tolerance showed significantly higher ABA sensitivity than drought-sensitive synthetic lines. In the hexaploid genetic background, therefore, weak association of ABA sensitivity with drought tolerance was observed. To study differences in gene expression patterns between stress-tolerant and -sensitive lines, levels of two Cor/Lea and three transcription factor gene transcripts were compared. The more tolerant accession of Ae. tauschii tended to accumulate more abundant transcripts of the examined genes than the sensitive accession under stress conditions. The expression patterns in the synthetic wheats seemed to be additive for parental lines exposed to drought and ABA treatments. However, the transcript levels of transcription factor genes in the synthetic wheats did not necessarily correspond to the postulated levels based on expression in parental lines. Allopolyploidization altered the expression levels of the stress-responsive genes in synthetic wheats.
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影响因子：
7.4
作者：
Jaglo, KR;Kleff, S;Thomashow, MF
通讯作者：
Thomashow, MF
影响因子：
6.9
作者：
Kobayashi, Fuminori;Maeta, Eri;Takumi, Shigeo
通讯作者：
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影响因子：
5.1
作者：
LANG, V;PALVA, ET
通讯作者：
PALVA, ET
影响因子：
5.4
作者：
Dvorak, J;Luo, MC;Zhang, HB
通讯作者：
Zhang, HB
影响因子：
2
作者：
Dudnikov, AJ;Kawahara, T
通讯作者：
Kawahara, T