The road to regenerative liver therapies: the triumphs, trials and tribulations.
The road to regenerative liver therapies: the triumphs, trials and tribulations.
复制标题
再生肝疗法的道路：胜利，试验和磨难。
DOI：
10.1016/j.biotechadv.2013.08.022
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发表时间：
2013-11-15
影响因子：
16
通讯作者：
Hu, Wei-Shou
中科院分区：
文献类型：
--
作者：
Raju, Ravali;Chau, David;Verfaillie, Catherine M.;Hu, Wei-Shou
肝脏是少数几个在肝衰竭或肝损伤后具有高再生能力的器官之一。肝脏的实质细胞，即肝细胞，参与了大部分的再生过程。因此，肝细胞移植是治疗肝损伤的另一种方法。然而，肝细胞的短缺和维持原代肝细胞的困难仍然是研究人员在肝细胞移植应用于临床实践之前必须克服的关键障碍。多能干细胞（PSCs）和诱导多能干细胞（iPSCs）的独特特性为产生足够的功能性肝细胞用于细胞治疗提供了一种替代方法。在这篇综述中，我们将简要介绍从PSCs和iPSCs分化肝细胞的现状。使用不同细胞来源（成体肝干细胞、肝母细胞、肝祖细胞等）的肝再生过程的研究将被详细描述，以及如何将这些知识应用于优化培养条件，以维持这些细胞向肝细胞的分化。随着干细胞衍生疗法的前景开始看起来更加可信，研究人员将需要解决我们必须克服的挑战，以便将干细胞研究转化为临床应用。
The liver is one of the few organs that possess a high capacity to regenerate after liver failure or liver damage. The parenchymal cells of the liver, hepatocytes, contribute to the majority of the regeneration process. Thus, hepatocyte transplantation presents an alternative method to treating liver damage. However, shortage of hepatocytes and difficulties in maintaining primary hepatocytes still remain key obstacles that researchers must overcome before hepatocyte transplantation can be used in clinical practice. The unique properties of pluripotent stem cells (PSCs) and induced pluripotent stem cells (iPSCs) have provided an alternative approach to generating enough functional hepatocytes for cellular therapy. In this review, we will present a brief overview on the current state of hepatocyte differentiation from PSCs and iPSCs. Studies of liver regenerative processes using different cell sources (adult liver stem cells, hepatoblasts, hepatic progenitor cells,etc.) will be described in detail as well as how this knowledge can be applied towards optimizing culture conditions for the maintenance and differentiation of these cells towards hepatocytes. As the outlook of stem cell-derived therapy begins to look more plausible, researchers will need to address the challenges we must overcome in order to translate stem cell research to clinical applications.
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影响因子：
2.7
作者：
Buschke DG;Squirrell JM;Fong JJ;Eliceiri KW;Ogle BM
通讯作者：
Ogle BM
影响因子：
4.8
作者：
Bakre, Manjiri Manohar;Hoi, Aina;Stanton, Lawrence W.
通讯作者：
Stanton, Lawrence W.
DOI：
10.1007/978-1-61779-468-1_6
发表时间：
2012-01-01
期刊：
LIVER STEM CELLS: METHODS AND PROTOCOLS
影响因子：
--
作者：
Banas, Agnieszka
通讯作者：
Banas, Agnieszka
影响因子：
0.7
作者：
De-Miguel, Maria P.;Arnalich-Montiel, Francisco;Nistal, Manuel
通讯作者：
Nistal, Manuel
影响因子：
1.7
作者：
Gerlach, JC;Zeilinger, K;Neuhaus, R
通讯作者：
Neuhaus, R