Cytoskeletal structure of skeletal muscle: identification of an intricate exosarcomeric microtubule lattice in slow- and fast-twitch muscle fibers.
Cytoskeletal structure of skeletal muscle: identification of an intricate exosarcomeric microtubule lattice in slow- and fast-twitch muscle fibers.
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骨骼肌的细胞骨架结构：慢肌纤维和快肌纤维中复杂的外肌微管晶格的识别。
DOI：
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发表时间：
1993
影响因子：
3.2
通讯作者：
P. Rogers
中科院分区：
文献类型：
--
作者：
S. Boudriau;M. Vincent;C. Côté;P. Rogers
我们用免疫化学定量和间接免疫荧光方法研究了成年大鼠慢抽动比目鱼肌和快抽动股外侧肌中微管的细胞含量、分布和组织。免疫印迹分析表明，比目鱼肌(主要是I型纤维)的α-微管蛋白的相对含量是股外侧肌浅层(主要是IIb型纤维)的1.7倍。这两种生理性肌肉类型都显示出肌浆间隙内微管的复杂排列，这些微管表现出倾斜、纵向和横向。任何一种特定取向的优势因肌肉组织切片的不同而显著不同。细胞核完全被致密的网状微管结构包围。这两种类型的肌肉纤维在肌膜下区域都有较高密度的微管。这些微管在轻微收缩的纤维中跟随肌膜的轮廓，并显示出细小的点状外观，表明分布受限。免疫荧光结果表明，微管与肌膜相关，因此可能构成肌纤维膜细胞骨架结构域的一部分。我们的结论是，成年哺乳动物骨骼肌纤维的微管网络与中间细丝一起构成了外壁细胞骨架格子结构域的骨骼成分，因此可能参与了肌肉纤维在收缩-松弛周期中各种细胞器的机械整合。
We used immunochemical quantification and indirect immunofluorescence to investigate the cell content, distribution, and organization of microtubules in adult rat slow-twitch soleus and fast-twitch vastus lateralis muscles. An immunoblotting assay demonstrated that the soleus muscle (primarily Type I fibers) was found to have a 1.7-fold higher relative content of alpha-tubulin compared with the superficial portion of the vastus lateralis muscle (primarily Type IIb fibers). Both physiological muscle types revealed a complex arrangement of microtubules which displayed oblique, longitudinal, and transverse orientations within the sarcoplasmic space. The predominance of any one particular orientation varied significantly from one muscle tissue section to another. Nuclei were completely surrounded by a dense net-like structure of microtubules. Both muscle fiber types were found to possess a higher density of microtubules in the subsarcolemmal region. These microtubules followed the contour of the sarcolemma in slightly contracted fibers and showed a fine punctate appearance indicative of a restricted distribution. The immunofluorescence results indicate that microtubules are associated with the sarcolemma and therefore may form a part of the membrane cytoskeletal domain of the muscle fiber. We conclude that the microtubule network of the adult mammalian skeletal muscle fiber constitutes a bone fide component of the exosarcomeric cytoskeletal lattice domain along with the intermediate filaments, and as such could therefore participate in the mechanical integration of the various organelles of the myofibers during the contraction-relaxation cycle.
紫杉醇和秋水酰胺对肌原纤维形成的影响。
DOI：
10.1073/pnas.79.21.6556
发表时间：
1982
影响因子：
11.1
作者：
Toyama,Y;Forry-Schaudies,S;Hoffman,B;Holtzer,H
通讯作者：
Holtzer,H
横纹肌中肌节相关的细胞骨架晶格。
DOI：
10.1007/978-1-4757-4723-2_10
发表时间：
1985
期刊：
Cell and muscle motility
影响因子：
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作者：
Wang,K
通讯作者：
Wang,K