Antioxidative and Anti-Melanogenic Activities of Bamboo Stems (Phyllostachys nigra variety henosis) via PKA/CREB-Mediated MITF Downregulation in B16F10 Melanoma Cells.
Antioxidative and Anti-Melanogenic Activities of Bamboo Stems (Phyllostachys nigra variety henosis) via PKA/CREB-Mediated MITF Downregulation in B16F10 Melanoma Cells.
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DOI：
10.3390/ijms19020409
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发表时间：
2018-01-30
影响因子：
5.6
通讯作者：
Shin HJ
中科院分区：
文献类型：
--
作者：
Choi MH;Jo HG;Yang JH;Ki SH;Shin HJ
黑竹竹类植物henosis是一种备受关注的竹种，其生物活性化合物（特别是叶）具有抗氧化、抗炎和抗肥胖的活性。竹茎中生物活性物质的抗氧化和抗黑色素生成活性的研究较少。在无细胞系统和B16 F10黑色素瘤细胞中研究了黑胡椒茎提取物的EtOAc级分（PN 3）的抗黑素生成和抗氧化活性。PN 3由类黄酮的混合物组成，如儿茶素、绿原酸、咖啡酸和对香豆酸。评价了其抗氧化活性（2，2-二苯基-1-苦基肼（DPPH）、2，2 ′-连氮基-双（3-乙基苯并噻唑啉-6-磺酸（ABTS））和清除羟自由基），以及对芬顿反应产生的活性氧（ROS）的抑制作用。PN 3体外对酪氨酸酶有抑制作用，半数抑制浓度（IC_（50））为240 μg/mL，体内细胞毒浓度> 100 μg/mL。通过PN 3处理，TYR、TRP-1和MITF的蛋白表达水平和mRNA转录水平以剂量依赖性方式降低。PN 3干扰细胞内蛋白激酶A（PKA）/cAMP反应元件结合蛋白（CREB）的磷酸化，表现出有效的抗黑素生成作用。PN 3可以抑制PKA/CREB和随后的小眼症相关转录因子（MITF）的降解，导致黑素生成酶和黑素生成的抑制，可能是由于黄酮类化合物的存在。这些特性使其成为美白化妆品添加剂的候选者。
Phyllostachys nigra var. henosis, a domestic bamboo species, has been attracting much attention; its bioactive compounds (especially in the leaf) show antioxidant, anti-inflammatory, and anti-obesity activities. Little information is available on the antioxidative and anti-melanogenetic activities of the bioactive compounds in bamboo stems. The anti-melanogenic and antioxidative activities of the EtOAc fraction (PN3) of a P. nigra stem extract were investigated in a cell-free system and in B16F10 melanoma cells. PN3 consisted of a mixture of flavonoids, such as catechin, chlorogenic acid, caffeic acid, and p-coumaric acid. The antioxidant activity (2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS)), and hydroxyl radical scavenging) was evaluated, as well as the inhibition of reactive oxygen species (ROS) produced by the Fenton reaction. PN3 showed in vitro tyrosinase inhibition activity with the half maximal inbihitory concentration (IC50) values of 240 μg/mL, and in vivo cytotoxic concentration ranges > 100 μg/mL. The protein expression levels and mRNA transcription levels of TYR, TRP-1, and MITF were decreased in a dose-dependent manner by the treatment with PN3. PN3 interfered with the phosphorylation of intracellular protein kinase A (PKA)/cAMP response element-binding protein (CREB), demonstrating potent anti-melanogenic effects. PN3 could inhibit PKA/CREB and the subsequent degradation of microphthalmia-associated transcription factor (MITF), resulting in the suppression of melanogenic enzymes and melanin production, probably because of the presence of flavonoid compounds. These properties make it a candidate as an additive to whitening cosmetics.
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影响因子：
6.7
作者：
Kim, DS;Park, SH;Park, KC
通讯作者：
Park, KC
DOI：
10.3390/molecules191117400
发表时间：
2014-10-29
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Brito A;Ramirez JE;Areche C;Sepúlveda B;Simirgiotis MJ
通讯作者：
Simirgiotis MJ
DOI：
10.1007/s11632-004-0001-9
发表时间：
2004-01-01
期刊：
Forestry Studies in China
影响因子：
--
作者：
Jun, M.;Tohru, U.;Li, J. Z.
通讯作者：
Li, J. Z.
影响因子：
4.8
作者：
Haycock, JW;Rowe, SJ;MacNeil, S
通讯作者：
MacNeil, S
影响因子：
2.3
作者：
Laitinen, J;Julkunen-Tiitto, R;Tahvanainen, J
通讯作者：
Tahvanainen, J