HPLC-UV-MS profiles of phenolic compounds and antioxidant activity of fruits from three citrus species consumed in Northern Chile.
HPLC-UV-MS profiles of phenolic compounds and antioxidant activity of fruits from three citrus species consumed in Northern Chile.
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DOI：
10.3390/molecules191117400
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发表时间：
2014-10-29
期刊：
影响因子：
--
通讯作者：
Simirgiotis MJ
中科院分区：
文献类型：
--
作者：
Brito A;Ramirez JE;Areche C;Sepúlveda B;Simirgiotis MJ
从三种柑橘，包括柑橘橙（品种皮卡和苏蒂尔）和柑橘x柠檬变种的果皮和可食用的果肉。热那亚广泛种植和消费的北方智利（I和II区）的酚类化合物和抗氧化活性进行了分析的第一次。建立了一种高效电喷雾质谱（HPLC-UV-ESI-MS）快速鉴定各种果皮和果汁中酚类化合物的方法。几种黄酮类化合物，包括一种山萘酚-O-己糖苷（峰16）和一种橙皮苷衍生物（峰22），三种槲皮素衍生物（峰4、19和36），五种异鼠李素衍生物（峰5、23、24、26和29）四种毛地黄黄酮衍生物（峰14、25、27和40），七种芹菜素衍生物（峰2、3、12、20、34、35和39），七种薯蓣皂苷衍生物（峰7-9、17、21、31和37），三种黄菊醇衍生物（峰10、18和30），以及四种杜鹃花醇衍生物（峰6、13、15和38）在阴性和阳性模式下使用全扫描质量测量和MSn碎片化鉴定。抗坏血酸含量较高的品种热那亚和苏蒂尔果肉（60.13 ± 1.28和56.53 ± 1.06毫克抗坏血酸每克干重，分别），而总酚含量较高的皮其次苏蒂尔果皮（34.59 ± 0.81和25.58 ± 1.02毫克/克GAE干重，分别）。Pica果皮的抗氧化能力也更高（DPPH测定中为10.34 ± 1.23 µg/mL，FRAP测定中为120.63 ± 2.45 µM trolox当量/g干重）。抗氧化剂特征与高多酚含量一起可以至少部分地支持果皮提取物作为营养补充剂的用途，特别是用作抗衰老产品。
Peels and edible pulp from three species of citrus including Citrus aurantifolia (varieties pica and sutil) and Citrus x lemon var. Genova widely cultivated and consumed in Northern Chile (I and II region) were analyzed for phenolic compounds and antioxidant activity for the first time. A high performance electrospray ionization mass spectrometry (HPLC-UV-ESI-MS) method was developed for the rapid identification of phenolics in extracts from peels and juices of all species. Several flavonoids including one kaempferol-O-hexoside (peak 16) and one hesperidin derivative (peak 22) three quercetin derivatives (peaks 4, 19 and 36), five isorhamnetin derivatives (peaks 5, 23, 24, 26 and 29) four luteolin derivatives (peaks 14, 25, 27 and 40), seven apigenin derivatives (peaks 2, 3, 12, 20, 34, 35 and 39), seven diosmetin derivatives (peaks 7–9, 17, 21, 31 and 37), three chrysoeriol derivatives (peaks 10, 18 and 30), and four eryodictiol derivatives (peaks 6, 13, 15 and 38) were identified in negative and positive mode using full scan mass measurements and MSn fragmentations. Ascorbic acid content was higher in the pulps of the varieties Genova and Sutil (60.13 ± 1.28 and 56.53 ± 1.06 mg ascorbic acid per g dry weight, respectively) while total phenolic content was higher in Pica peels followed by Sutil peels (34.59 ± 0.81 and 25.58 ± 1.02 mg/g GAE dry weight, respectively). The antioxidant capacity was also higher for Pica peels (10.34 ± 1.23 µg/mL in the DPPH assay and 120.63 ± 2.45 µM trolox equivalents/g dry weight in the FRAP assay). The antioxidant features together with the high polyphenolic contents can support at least in part, the usage of the peel extracts as nutraceutical supplements, especially to be used as anti-ageing products.
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DOI：
10.1016/s1044-0305(01)00226-4
发表时间：
2001-06-01
影响因子：
3.2
作者：
Fabre, N;Rustan, I;Quetin-Leclercq, J
通讯作者：
Quetin-Leclercq, J
影响因子：
6.1
作者：
Abad-Garcia, Beatriz;Garmon-Lobato, Sergio;Vicente, Francisca
通讯作者：
Vicente, Francisca
影响因子：
8.8
作者：
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通讯作者：
Chen, KunSong
影响因子：
5.9
作者：
Dahmoune, Farid;Boulekbache, Lila;Madani, Khodir
通讯作者：
Madani, Khodir
快速分辨液相色谱-三重四极杆电喷雾串联质谱法同时测定陈皮中六种生物活性黄酮类成分
影响因子：
8.8
作者：
Liu, E-Hu;Zhao, Pan;Li, Ping
通讯作者：
Li, Ping