Vimentin intermediate filaments provide structural stability to the mammalian Golgi complex.
Vimentin intermediate filaments provide structural stability to the mammalian Golgi complex.
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DOI：
10.1242/jcs.260577
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发表时间：
2023-10-15
影响因子：
4
通讯作者：
Lowe, Martin
中科院分区：
文献类型：
--
作者：
Vitali, Teresa;Sanchez-Alvarez, Rosa;Witkos, Tomasz M.;Bantounas, Ioannis;Cutiongco, Marie F. A.;Dudek, Michal;Yan, Guanhua;Mironov, Alexander A.;Swift, Joe;Lowe, Martin
高尔基复合体由位于大多数脊椎动物细胞核周区域的一条相互连接的层叠池膜带组成。这种细胞器的位置和形态取决于与微管和肌动蛋白细胞骨架的相互作用。相比之下，我们知道相对较少的高尔基复合体与中间丝（IF）的关系。在这项研究中，我们表明，高尔基体是在物理上接近波形蛋白IF在培养的小鼠和人类细胞。我们还表明，反式高尔基体网络卷曲螺旋蛋白GORAB可以与波形蛋白IF物理关联。波形蛋白和/或GORAB的缺失对稳态下的高尔基体结构有适度的影响。在波形蛋白敲除细胞中，用布雷菲德菌素A或诺考达唑进行化学破坏后，高尔基体经历了更快的解体，而药物洗脱后的重组速度较慢。此外，当细胞在高刚度水凝胶上培养时，波形蛋白的损失导致高尔基带完整性降低，这因GORAB的损失而加剧。这些结果表明，波形蛋白IF有助于高尔基复合体的结构稳定性，并建议在这个过程中的作用GORAB。总结：波形蛋白中间丝与反式高尔基体网络蛋白GORAB相关联，并为哺乳动物细胞中的高尔基体复合体提供结构稳定性。
The Golgi complex comprises a connected ribbon of stacked cisternal membranes localized to the perinuclear region in most vertebrate cells. The position and morphology of this organelle depends upon interactions with microtubules and the actin cytoskeleton. In contrast, we know relatively little about the relationship of the Golgi complex with intermediate filaments (IFs). In this study, we show that the Golgi is in close physical proximity to vimentin IFs in cultured mouse and human cells. We also show that the trans-Golgi network coiled-coil protein GORAB can physically associate with vimentin IFs. Loss of vimentin and/or GORAB had a modest effect upon Golgi structure at the steady state. The Golgi underwent more rapid disassembly upon chemical disruption with brefeldin A or nocodazole, and slower reassembly upon drug washout, in vimentin knockout cells. Moreover, loss of vimentin caused reduced Golgi ribbon integrity when cells were cultured on high-stiffness hydrogels, which was exacerbated by loss of GORAB. These results indicate that vimentin IFs contribute to the structural stability of the Golgi complex and suggest a role for GORAB in this process. Summary: Vimentin intermediate filaments associate with the trans-Golgi network protein GORAB and provide structural stability to the Golgi complex in mammalian cells.
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影响因子：
3.3
作者：
Copeland SJ;Thurston SF;Copeland JW
通讯作者：
Copeland JW
影响因子：
6.6
作者：
Gao, YS;Vrielink, A;Sztul, E
通讯作者：
Sztul, E
影响因子：
64.5
作者：
FRANKE, WW;HERGT, M;GRUND, C
通讯作者：
GRUND, C
影响因子：
3.7
作者：
Burman JL;Hamlin JN;McPherson PS
通讯作者：
McPherson PS
DOI：
10.1126/science.1171643
发表时间：
2009-06-26
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Discher DE;Mooney DJ;Zandstra PW
通讯作者：
Zandstra PW