Characterization of a set of abdominal neuroendocrine cells that regulate stress physiology using colocalized diuretic peptides in Drosophila.
Characterization of a set of abdominal neuroendocrine cells that regulate stress physiology using colocalized diuretic peptides in Drosophila.
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DOI：
10.1007/s00018-017-2682-y
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发表时间：
2018-03
期刊：
影响因子：
--
通讯作者：
Nässel DR
中科院分区：
文献类型：
--
作者：
Zandawala M;Marley R;Davies SA;Nässel DR
已知多种神经肽调节果蝇体内的水和离子平衡。这些肽中的几种还具有生理学和行为学中的其他功能。例如促肾上腺皮质激素释放因子样利尿激素（利尿激素44; DH 44）和白细胞激肽（LK），这两种激素均诱导马氏管（MT）分泌液体，但也调节应激反应、进食、昼夜节律活动和其他行为。在这里，我们研究了LK和DH 44信号系统之间的功能关系。DH 44和LK肽仅共定位于一组腹部神经分泌细胞（ABLK）中。靶向敲低ABLK中的这些肽中的每一种导致对干燥、饥饿和离子应激的抗性增加。食物摄取通过DH 44的敲低而减少，但不通过LK的敲低而减少，并且保水性仅通过LK敲低而增加。因此，这两种共定位的肽显示出相似的全身作用，但在摄食和身体水分保持的调节方面不同。我们还证明了DH 44和LK对MT的液体分泌具有相加作用。可能的是，共定位的肽从ABLK共释放到循环中并作用于肾小管，在那里它们靶向不同的细胞类型和信号系统以调节利尿和应激耐受。另外的靶点似乎对两种肽中的每一种都是特异性的，并且有助于调节摄食和水保持。我们的数据表明，ABLK和激素的作用是足够的许多已知的DH 44和LK功能，并在中枢神经系统中的其余神经元发挥其他功能的作用。本文的在线版本（doi：10.1007/s 00018 -017-2682-y）包含补充材料，可供授权用户使用。
Multiple neuropeptides are known to regulate water and ion balance in Drosophila melanogaster. Several of these peptides also have other functions in physiology and behavior. Examples are corticotropin-releasing factor-like diuretic hormone (diuretic hormone 44; DH44) and leucokinin (LK), both of which induce fluid secretion by Malpighian tubules (MTs), but also regulate stress responses, feeding, circadian activity and other behaviors. Here, we investigated the functional relations between the LK and DH44 signaling systems. DH44 and LK peptides are only colocalized in a set of abdominal neurosecretory cells (ABLKs). Targeted knockdown of each of these peptides in ABLKs leads to increased resistance to desiccation, starvation and ionic stress. Food ingestion is diminished by knockdown of DH44, but not LK, and water retention is increased by LK knockdown only. Thus, the two colocalized peptides display similar systemic actions, but differ with respect to regulation of feeding and body water retention. We also demonstrated that DH44 and LK have additive effects on fluid secretion by MTs. It is likely that the colocalized peptides are coreleased from ABLKs into the circulation and act on the tubules where they target different cell types and signaling systems to regulate diuresis and stress tolerance. Additional targets seem to be specific for each of the two peptides and subserve regulation of feeding and water retention. Our data suggest that the ABLKs and hormonal actions are sufficient for many of the known DH44 and LK functions, and that the remaining neurons in the CNS play other functional roles. The online version of this article (doi:10.1007/s00018-017-2682-y) contains supplementary material, which is available to authorized users.
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影响因子：
64.5
作者：
Asahina K;Watanabe K;Duistermars BJ;Hoopfer E;González CR;Eyjólfsdóttir EA;Perona P;Anderson DJ
通讯作者：
Anderson DJ
影响因子：
4.5
作者：
Chen J;Reiher W;Hermann-Luibl C;Sellami A;Cognigni P;Kondo S;Helfrich-Förster C;Veenstra JA;Wegener C
通讯作者：
Wegener C
DOI：
10.1152/ajpregu.00584.2001
发表时间：
2002-05-01
影响因子：
2.8
作者：
Kean, L;Cazenave, W;Dow, JAT
通讯作者：
Dow, JAT
影响因子：
9.2
作者：
Brogiolo, W;Stocker, H;Hafen, E
通讯作者：
Hafen, E
影响因子：
3.6
作者：
de Haro, Maria;Al-Ramahi, Ismael;Herrero, Pilar
通讯作者：
Herrero, Pilar