Evaluation of the ERETIC method as an improved quantitative reference for 1H HR-MAS spectroscopy of prostate tissue.
Evaluation of the ERETIC method as an improved quantitative reference for 1H HR-MAS spectroscopy of prostate tissue.
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DOI：
10.1002/mrm.21808
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发表时间：
2009-03
影响因子：
3.3
通讯作者：
Kurhanewicz, John
中科院分区：
文献类型：
--
作者：
Albers, Mark J.;Butler, Thomas N.;Rahwa, Iman;Bao, Nguyen;Keshari, Kayvan R.;Swanson, Mark G.;Kurhanewicz, John
The Electronic REference To access In vivo Concentrations (ERETIC) method was applied to 1H HR-MAS spectroscopy. The accuracy, precision, and stability of ERETIC as a quantitative reference were evaluated in solution and human prostate tissue samples. For comparison, the reliability of 3-(trimethylsilyl)propionic-2,2,3,3-d4 acid (TSP) as a quantitation reference was also evaluated. The ERETIC and TSP peak areas were found to be stable in solution over the short-term and long-term, with long-term relative standard deviations (RSDs) of 4.10% and 2.60%, respectively. Quantification of TSP in solution using the ERETIC peak as a reference and a calibrated, rotor-dependent conversion factor yielded results with a precision ≤2.9% and an accuracy error ≤4.2% when compared with the expected values. The ERETIC peak area reproducibility was superior to TSP’s reproducibility, corrected for mass, in both prostate surgical and biopsy samples (4.53% vs. 21.2% and 3.34% vs. 31.8%, respectively). Furthermore, the tissue TSP peaks exhibited only 27.5% of the expected area, which would cause an overestimation of metabolite concentrations if used as a reference. The improved quantification accuracy and precision provided by ERETIC may enable the detection of smaller metabolic differences that may exist between individual tissue samples and disease states.
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