Riboflavin Level Manipulates the Successive Developmental Sequences in Aspergillus nidulans
Riboflavin Level Manipulates the Successive Developmental Sequences in Aspergillus nidulans
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核黄素水平控制构巢曲霉的连续发育序列
DOI：
10.1007/s00284-014-0723-4
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发表时间：
2015-01
影响因子：
2.6
通讯作者：
Lu Ling
中科院分区：
文献类型：
--
作者：
Zheng Hailin;Zhang Shenghua;Zhang Shizhu;Lu Ling
营养型标记在真菌遗传分析中是有用的。在营养缺陷型标记中，riboB 2是许多实验室菌株中最常用的标记之一。然而，在构巢曲霉中的riboB 2突变体赋予自交不育性，并且因此当两个亲本菌株在标准方案下均具有riboB 2营养缺陷型标记时，不能通过异交形成杂交闭囊酵母。探讨核黄素在不同发育阶段的作用。在nidulans中，监测核黄素的有限浓度。标准培养基配方中常用的核黄素剂量（2.5 μg/ml）足以使核黄素营养缺陷型riboB 2突变体（分别为0.02和0.5 μg/ml）在A. nidulans。然而，剂量不足以支持成熟闭囊壳的形成。此外，在标准培养基上添加25 μg/ml核黄素可以恢复riboB 2突变体的自交不育缺陷，尽管所需的核黄素浓度在A. nidulans。最重要的是，riboB 2突变体之间的异交也可以通过在性发育阶段提供核黄素来实现。本研究结果突出了营养缺陷型标记在真菌发育中的潜在作用，提高了A. nidulans。
Auxotrophic markers are useful in fungal genetic analysis. Among the auxotrophic markers, riboB2 is one of the most commonly used markers in many laboratory strains. However, riboB2 mutants in Aspergillus nidulans confer self-sterility and thus are unable to form hybrid cleistothecia by outcross when both parent strains harbor riboB2 auxotrophic marker under the standard protocol. To assess the role of riboflavin during the different developmental stages of A. nidulans, the limited concentrations of riboflavin were monitored. The commonly used dosage of riboflavin (2.5 µg/ml) in the standard medium recipe is enough for hyphal growth and conidiation in the riboflavin auxotrophic riboB2 mutants (enough at 0.02 and 0.5 μg/ml, respectively) in A. nidulans. However, the dosage is not enough to support mature cleistothecium formation. Furthermore, the self-sterile defects in riboB2 mutants on standard medium could be restored by the addition of 25 μg/ml riboflavin, although the required riboflavin concentrations are varied in different genotype strains in A. nidulans. Most importantly, the outcross between riboB2 mutants could also be achieved by the supply of riboflavin in the sexual developmental stage. Our results highlight the potential roles of auxotrophic markers in the development of fungi and improve the efficiency of the genetic analysis in A. nidulans.
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影响因子：
5.1
作者：
K. Chae;Dong-Min Han;K. Han
通讯作者：
K. Chae;Dong-Min Han;K. Han
影响因子：
4.9
作者：
E. Kuttin;W. Kaplan;H. Scholer;H. Burtscher;H. Köhler
通讯作者：
E. Kuttin;W. Kaplan;H. Scholer;H. Burtscher;H. Köhler
影响因子：
3.6
作者：
AGUIRRE, J
通讯作者：
AGUIRRE, J
影响因子：
--
作者：
Adelbert Bacher;S. Eberhardt;W. Eisenreich;Markus Fischer;S. Herz;B. Illarionov;K. Kis;G. Richter
通讯作者：
Adelbert Bacher;S. Eberhardt;W. Eisenreich;Markus Fischer;S. Herz;B. Illarionov;K. Kis;G. Richter
影响因子：
3.6
作者：
M. Vallim;K. Miller;B. Miller
通讯作者：
M. Vallim;K. Miller;B. Miller