The Effects of Consuming White Button Mushroom Agaricus bisporus on the Brain and Liver Metabolome Using a Targeted Metabolomic Analysis.
The Effects of Consuming White Button Mushroom Agaricus bisporus on the Brain and Liver Metabolome Using a Targeted Metabolomic Analysis.
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DOI：
10.3390/metabo11110779
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发表时间：
2021-11-15
期刊：
影响因子：
4.1
通讯作者：
Urban JF Jr
中科院分区：
文献类型：
--
作者：
Solano-Aguilar GI;Lakshman S;Jang S;Gupta R;Molokin A;Schroeder SG;Gillevet PM;Urban JF Jr
对喂食添加了双孢蘑菇（WBM）饲料的猪的组织进行了靶向代谢组学分析，以确定其对肝脏和大脑代谢组的影响。31头猪分别单独喂食生长饲料，或在饲料中添加3份或6份冻干的双孢蘑菇，持续6周。使用靶向液相色谱 - 质谱联用技术（LC - MS）结合化学相似性富集分析（ChemRICH）对组织代谢组进行分析，并与双孢蘑菇诱导的粪便微生物组组成变化相关联。结果表明，双孢蘑菇能够差异调节健康猪的肝脏、大脑皮层和海马体中的代谢物。在甘油磷脂中，喂食6份双孢蘑菇的猪的海马体中烷基 - 酰基 - 磷脂酰胆碱（PC - O 40:3）有所增加。在肝脏中检测到甘油磷脂和鞘脂更广泛的变化，喂食两种双孢蘑菇饲料的猪的几种脂质种类减少，但喂食6份双孢蘑菇的猪的皮层中作为神经递质前体的氨基酸增加。代谢组学变化与微生物组中冷弯菌科、毛螺菌科、火色杆菌科和瘤胃球菌科的丰度增加呈正相关，这表明双孢蘑菇也能对组织代谢物组成产生积极影响。
A targeted metabolomic analysis was performed on tissues derived from pigs fed diets supplemented with white button mushrooms (WBM) to determine the effect on the liver and brain metabolome. Thirty-one pigs were fed a grower diet alone or supplemented with either three or six servings of freeze-dried WBM for six weeks. Tissue metabolomes were analyzed using targeted liquid chromatography-mass spectrometry (LC-MS) combined with chemical similarity enrichment analysis (ChemRICH) and correlated to WBM-induced changes in fecal microbiome composition. Results indicated that WBM can differentially modulate metabolites in liver, brain cortex and hippocampus of healthy pigs. Within the glycero-phospholipids, there was an increase in alkyl-acyl-phosphatidyl-cholines (PC-O 40:3) in the hippocampus of pigs fed six servings of WBM. A broader change in glycerophospholipids and sphingolipids was detected in the liver with a reduction in several lipid species in pigs fed both WBM diets but with an increase in amino acids known as precursors of neurotransmitters in the cortex of pigs fed six servings of WBM. Metabolomic changes were positively correlated with increased abundance of Cryomorphaceae, Lachnospiraceae, Flammeovirgaceae and Ruminococcaceae in the microbiome suggesting that WBM can also positively impact tissue metabolite composition.
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影响因子：
10.6
作者：
Bruce-Keller AJ;Salbaum JM;Luo M;Blanchard E 4th;Taylor CM;Welsh DA;Berthoud HR
通讯作者：
Berthoud HR
猪翻译研究数据库：基于手动的，基因组学和基于蛋白质组学的研究资源。
影响因子：
4.4
作者：
Dawson HD;Chen C;Gaynor B;Shao J;Urban JF Jr
通讯作者：
Urban JF Jr
DOI：
10.1615/intjmedmushrooms.2020033807
发表时间：
2020-01-01
影响因子：
1.2
作者：
Asad, Farhan;Anwar, Haseeb;Sohail, Muhammad Umar
通讯作者：
Sohail, Muhammad Umar
影响因子：
5.5
作者：
Borg, Melissa L.;Omran, Simin Fallah;Watt, Matthew J.
通讯作者：
Watt, Matthew J.
影响因子：
4.3
作者：
Fonteh AN;Chiang AJ;Arakaki X;Edminster SP;Harrington MG
通讯作者：
Harrington MG