EGF Functionalized Polymer-Coated Gold Nanoparticles Promote EGF Photostability and EGFR Internalization for Photothermal Therapy
EGF Functionalized Polymer-Coated Gold Nanoparticles Promote EGF Photostability and EGFR Internalization for Photothermal Therapy
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EGF 功能化聚合物涂层金纳米粒子促进 EGF 光稳定性和 EGFR 内化用于光热治疗
作者：
C. Silva;S. Petersen;C. Reis;P. Ríjo;J. Molpeceres;A. Fernandes;Odete Gonçalves;A. Gomes;I. Correia;H. Vorum;M. Neves
功能化纳米载体在光热治疗肿瘤消融中的应用受到广泛关注。这种应用的成功取决于系统的治疗效率和生物相容性，但也取决于结合蛋白的稳定性和生物识别性。本研究旨在探讨EGF功能化聚合物包覆金纳米颗粒促进EGF光稳定性和EGFR内化的假设，使这些共轭颗粒适合光热治疗。共轭金纳米粒子（100-200 nm）在近红外（650-900 nm）范围内显示出等离子体吸收带，最适合光热治疗应用。采用稳态和时间分辨荧光光谱技术研究了温度、聚合物包覆金纳米粒子和UVB光（295nm）对EGF荧光性质的影响。EGF的荧光特性，包括色氨酸和Tyr光产物的形成，受温度和激发光强度的调节。当EGF暴露在UVB光下时，聚合物包覆的金纳米粒子的存在减少甚至避免了Trp和Tyr光产物的形成，从而保护了EGF的结构和功能。在正常样人角质形成细胞中进行的共轭纳米颗粒细胞毒性研究显示，细胞活力的浓度依赖性降低（0-25%）。此外，共轭纳米颗粒可以激活和诱导人肺癌细胞中过表达的表皮生长因子受体内化。综上所述，本研究开发的结合表皮生长因子并包被生物聚合物的金纳米颗粒在近红外光热治疗中具有潜在的应用前景，可以有效地破坏实体肿瘤，减少对健康组织的损伤。
The application of functionalized nanocarriers on photothermal therapy for cancer ablation has wide interest. The success of this application depends on the therapeutic efficiency and biocompatibility of the system, but also on the stability and biorecognition of the conjugated protein. This study aims at investigating the hypothesis that EGF functionalized polymer-coated gold nanoparticles promote EGF photostability and EGFR internalization, making these conjugated particles suitable for photothermal therapy. The conjugated gold nanoparticles (100–200 nm) showed a plasmon absorption band located within the near-infrared range (650–900 nm), optimal for photothermal therapy applications. The effects of temperature, of polymer-coated gold nanoparticles and of UVB light (295nm) on the fluorescence properties of EGF have been investigated with steady-state and time-resolved fluorescence spectroscopy. The fluorescence properties of EGF, including the formation of Trp and Tyr photoproducts, is modulated by temperature and by the intensity of the excitation light. The presence of polymeric-coated gold nanoparticles reduced or even avoided the formation of Trp and Tyr photoproducts when EGF is exposed to UVB light, protecting this way the structure and function of EGF. Cytotoxicity studies of conjugated nanoparticles carried out in normal-like human keratinocytes showed small, concentration dependent decreases in cell viability (0–25%). Moreover, conjugated nanoparticles could activate and induce the internalization of overexpressed Epidermal Growth Factor Receptor in human lung carcinoma cells. In conclusion, the gold nanoparticles conjugated with Epidermal Growth Factor and coated with biopolymers developed in this work, show a potential application for near infrared photothermal therapy, which may efficiently destroy solid tumours, reducing the damage of the healthy tissue.
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DOI：
--
发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Matrisian,LM;Planck,SR;Magun,BE
通讯作者：
Magun,BE
影响因子：
--
作者：
Kurnasov, O;Goral, V;Begley, TP
通讯作者：
Begley, TP
影响因子：
4.9
作者：
Qhattal HS;Liu X
通讯作者：
Liu X
DOI：
10.2217/nnm.12.160
发表时间：
2012-12
期刊：
Nanomedicine (London, England)
影响因子：
--
作者：
Master AM;Sen Gupta A
通讯作者：
Sen Gupta A
通过等电聚焦纯化的 125I-表皮生长因子的结合、内化和细胞内加工。
DOI：
10.1016/0006-291x(82)91866-6
发表时间：
1982
影响因子：
3.1
作者：
Magun,BE;Planck,SR;Matrisian,LM;Finch,JS
通讯作者：
Finch,JS