Visualizing the entire cortical myelination pattern in marmosets with magnetic resonance imaging.
Visualizing the entire cortical myelination pattern in marmosets with magnetic resonance imaging.
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DOI：
10.1016/j.jneumeth.2009.08.022
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发表时间：
2009-12-15
影响因子：
3
通讯作者：
Silva, Afonso C.
中科院分区：
文献类型：
--
作者：
Bock, Nicholas A.;Kocharyan, Ara;Liu, Junjie V.;Silva, Afonso C.
关键词：
骨髓结构，即整个大脑皮层的髓磷脂密度模式，长期以来一直在组织学切片中可视化，以识别皮层的不同解剖区域。在人类中，二维 (2D) 磁共振成像 (MRI) 已用于可视化皮层选定区域的骨髓结构，例如初级视觉皮层中的 Gennari 条纹和初级听觉皮层中的 Heschl 回。在这里，我们研究了使用基于纵向弛豫时间 (T1) 的 MRI 对比来可视化普通狨猴 (Callithrix jacchus) 整个皮层的体内骨髓结构，狨猴是一种小型非人类灵长类动物，在神经科学和神经生物学研究中变得越来越重要。通过定量 T1 映射，我们发现髓磷脂含量高的皮质区域中 7 特斯拉的 T1 比髓磷脂含量低的区域中的 T1 短 15%。为了最大化皮质髓鞘形成模式成像的 T1 对比度，我们优化了磁化准备的快速采集梯度回波 (MP-RAGE) 序列。在使用该序列在体内制作的全脑 3D T1 加权图像中，我们确定了六个具有高髓鞘形成的主要皮质区域，并通过髓磷脂染色的组织学切片证实了结果。我们还确定了骨髓结构的几个微妙特征，显示了我们技术的敏感性。对整个皮层的骨髓结构进行成像的能力可能有助于研究与发育和神经元可塑性相关的皮层地形的纵向变化，以及指导和确认功能测量的位置。
Myeloarchitecture, the pattern of myelin density across the cerebral cortex, has long been visualized in histological sections to identify distinct anatomical areas of the cortex. In humans, two-dimensional (2D) magnetic resonance imaging (MRI) has been used to visualize myeloarchitecture in select areas of the cortex, such as the stripe of Gennari in the primary visual cortex and Heschl’s gyrus in the primary auditory cortex. Here, we investigated the use of MRI contrast based on longitudinal relaxation time (T1) to visualize myeloarchitecture in vivo over the entire cortex of the common marmoset (Callithrix jacchus), a small non-human primate that is becoming increasingly important in neuroscience and neurobiology research. Using quantitative T1 mapping, we found that T1 at 7 Tesla in a cortical region with a high myelin content was 15% shorter than T1 in a region with a low myelin content. To maximize this T1 contrast for imaging cortical myelination patterns, we optimized a magnetization-prepared rapidly-acquired gradient echo (MP-RAGE) sequence. In whole-brain, 3D T1-weighted images made in vivo with the sequence, we identified six major cortical areas with high myelination and confirmed the results with histological sections stained for myelin. We also identified several subtle features of myeloarchitecture, showing the sensitivity of our technique. The ability to image myeloarchitecture over the entire cortex may prove useful in studies of longitudinal changes of the topography of the cortex associated with development and neuronal plasticity, as well as for guiding and confirming the location of functional measurements.
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