Expression of pluripotency master regulators during two key developmental transitions: EGA and early lineage specification in the bovine embryo.
Expression of pluripotency master regulators during two key developmental transitions: EGA and early lineage specification in the bovine embryo.
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DOI：
10.1371/journal.pone.0034110
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Duranthon V
中科院分区：
文献类型：
--
作者：
Khan DR;Dubé D;Gall L;Peynot N;Ruffini S;Laffont L;Le Bourhis D;Degrelle S;Jouneau A;Duranthon V
多能性基因与小鼠胚胎基因组激活（EGA）和多能谱系规范有关。此外，它们的表达水平与胚胎足月发育有关。然而，在牛中，关于多能性基因在这些过程中的动态信息很少。在这项研究中，我们绘制了牛卵母细胞和早期胚胎中多能基因（OCT4， SOX2和NANOG）转录本和蛋白质的定量和/或定性时空表达模式及其下游靶点的mRNA水平。此外，为了将这些基因的表达模式与足月发育潜力联系起来，我们使用了体外相似但足月发育率不同的克隆胚胎。我们的研究结果证实：首先，尽管OCT4和SOX2的转录本存在，但核心的三联多能性基因可能与牛EGA无关，因为它们的蛋白在EGA前期未被检测到。其次，与OCT4相比，SOX2和NANOG的转录本和蛋白质的早期ICM规范使它们成为牛多能谱系规范的合适候选者。第三，低发育潜能的胚胎转录率较高；然而，多能性基因之间的不稳定平衡得以维持。这种平衡预示着正常的体外发育，但可能较高的转录率在后期扰乱了它，从而取消了长期发育。
Pluripotency genes are implicated in mouse embryonic genome activation (EGA) and pluripotent lineage specification. Moreover, their expression levels have been correlated with embryonic term development. In bovine, however, little information is available about dynamics of pluripotency genes during these processes. In this study, we charted quantitative and/or qualitative spatio-temporal expression patterns of transcripts and proteins of pluripotency genes (OCT4, SOX2 and NANOG) and mRNA levels of some of their downstream targets in bovine oocytes and early embryos. Furthermore, to correlate expression patterns of these genes with term developmental potential, we used cloned embryos, having similar in vitro but different full term development rates. Our findings affirm: firstly, the core triad of pluripotency genes is probably not implicated in bovine EGA since their proteins were not detected during pre-EGA phase, despite the transcripts for OCT4 and SOX2 were present. Secondly, an earlier ICM specification of transcripts and proteins of SOX2 and NANOG makes them pertinent candidates of bovine pluripotent lineage specification than OCT4. Thirdly, embryos with low term development potential have higher transcription rates; nevertheless, precarious balance between pluripotency genes is maintained. This balance presages normal in vitro development but, probably higher transcription rate disturbs it at later stage that abrogates term development.
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