Complexation of bisphosphonates with ytterbium(III): application of phosphate and ATP detection assay based on Yb(3+)-pyrocatechol violet.
Complexation of bisphosphonates with ytterbium(III): application of phosphate and ATP detection assay based on Yb(3+)-pyrocatechol violet.
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DOI：
10.1016/j.jinorgbio.2009.09.006
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发表时间：
2009-12
影响因子：
3.9
通讯作者：
Crans, Debbie C.
中科院分区：
文献类型：
--
作者：
Gaidamauskas, Ernestas;Parker, Helen;Kashemirov, Boris A.;Holder, Alvin A.;Saejueng, Kanokkarn;McKenna, Charles E.;Crans, Debbie C.
关键词：
The coordination chemistry of bisphosphonates with Yb3+ was investigated to evaluate the potential of the UV-Vis based detection method using the Yb3+-pyrocatechol complexation reaction as a sensor for bisphosphonates. The complexation chemistry of Yb3+ with phosphate and ATP analogs was previously described (E. Gaidamauskas et al J. Biol. Inorg. Chem. 13 (2008) 1291-1299), and we here study the complexation chemistry of bisphosphonates in this system. The spectrophotometric assay yields direct evidence for formation of a 4:3 metal to ligand complex at neutral pH. Direct evidence for Yb3+ : methylenebis(phosphonate) complexes with 1:1 and 1:2 stoichiometry was also obtained by potentiometry at acidic and basic pH. Direct evidence for complex formation was obtained using 1H NMR spectroscopy although the stoichiometry was not accessed at neutral pH. Our results suggest that the spectroscopic observation of the YbPV complex can be used to conveniently measure concentrations of bisphosphonates down to 2-3 μM .
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影响因子：
3.9
作者：
CLAESSENS, RAMJ;VANDERLINDEN, JGM
通讯作者：
VANDERLINDEN, JGM
影响因子：
4.1
作者：
Lovdahl, MJ;Pietrzyk, DJ
通讯作者：
Pietrzyk, DJ
影响因子：
4.1
作者：
QIN, XZ;TSAI, EW;IP, DP
通讯作者：
IP, DP
影响因子：
4.6
作者：
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通讯作者：
Smith, Rhett C.
影响因子：
2.8
作者：
Nash, KL;Rogers, RD;Zhang, J
通讯作者：
Zhang, J