IP6 reduces colorectal cancer metastasis by mediating the interaction of gut microbiota with host genes.
IP6 reduces colorectal cancer metastasis by mediating the interaction of gut microbiota with host genes.
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IP6通过介导肠道微生物群与宿主基因的相互作用减少结直肠癌转移。
DOI：
10.3389/fnut.2022.979135
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发表时间：
2022
影响因子：
5
通讯作者：
Li, Hui
中科院分区：
文献类型：
--
作者：
Lan, Tong-Tong;Song, Yang;Liu, Xiao-Han;Liu, Cui-Ping;Zhao, Hui-Chao;Han, Yi-Sa;Wang, Chu-Hui;Yang, Ning;Xu, Zhen;Tao, Meng;Li, Hui
肌醇六磷酸（IP6）是一种广泛存在于谷物和豆类中的植物化学物质，具有抗癌作用。然而，IP6通过宿主基因、肠道菌群及其相互作用抑制结直肠癌转移的机制尚不清楚。本研究采用16S rRNA测序技术研究IP6对结肠直肠癌小鼠原位移植模型肠道菌群的影响。利用转录组研究转移过程中宿主基因的变化及其与肠道菌群的关系。结果表明，模型小鼠的肠道菌群组成与正常小鼠有显著差异。经IP6干预后，β多样性部分趋于恢复正常水平。ip6处理后，helveticus乳杆菌和乳酸乳球菌的回收率最高。富集分析显示，与模型组相比，IP6处理后细胞因子-细胞因子受体相互作用信号通路富集评分降低。进一步分析该通路的差异表达基因（DEGs）发现，IP6降低了与肝转移面积相关的Tnfrsf1b基因的表达，且Tnfrsf1b基因与helveticus乳杆菌的相对丰度呈负相关。我们的研究结果表明，宿主基因、微生物组及其相互作用可能是IP6干预结直肠癌转移机制的有希望的靶点。
Inositol hexaphosphate (IP6) is a phytochemical widely found in grains and legumes that plays an anti-cancer role. However, the mechanism underlying the inhibition of colorectal cancer metastasis by IP6 through host genes, gut microbiota, and their interactions remain elusive. In this study, 16S rRNA sequencing was used to study the effect of IP6 on gut microbiota in an orthotopic transplantation model of colorectal cancer mice. The transcriptome was used to study the changes of host genes in metastasis and the relationship with gut microbiota. The results showed that the gut microbiota composition of model mice was significantly different from that of normal mice. The beta diversity partly tended to return to the normal level after IP6 intervention. Especially, Lactobacillus helveticus and Lactococcus lactis were recovered after IP6-treated. Enrichment analysis showed that the enrichment score of the Cytokine-Cytokine receptor interaction signal pathway decreased after IP6 treatment compared to the model group. Further analysis of differentially expressed genes (DEGs) in this pathway showed that IP6 reduced the expression of the Tnfrsf1b gene related to the area of liver metastasis, and the Tnfrsf1b gene was negatively correlated with the relative abundance of Lactobacillus helveticus. Our results presented that host gene, microbiome and their interaction may serve as promising targets for the mechanism of IP6 intervention in colorectal cancer metastasis.
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DOI：
10.1158/1541-7786.mcr-10-0210
发表时间：
2011-12
期刊：
Molecular cancer research : MCR
影响因子：
--
作者：
Hamilton KE;Simmons JG;Ding S;Van Landeghem L;Lund PK
通讯作者：
Lund PK
DOI：
10.1136/bmj.c5504
发表时间：
2010-10-26
期刊：
BMJ (Clinical research ed.)
影响因子：
--
作者：
Kirkegaard H;Johnsen NF;Christensen J;Frederiksen K;Overvad K;Tjønneland A
通讯作者：
Tjønneland A
影响因子：
2.8
作者：
Eun, Chang Soo;Kim, Yong Seok;Park, Yoon Kyung
通讯作者：
Park, Yoon Kyung
影响因子：
3.8
作者：
Hackl C;Neumann P;Gerken M;Loss M;Klinkhammer-Schalke M;Schlitt HJ
通讯作者：
Schlitt HJ
影响因子：
4
作者：
Liu, Xiaohan;Liu, Cuiping;Song, Yang
通讯作者：
Song, Yang