MicroRNA-133a-3p inhibits cell proliferation, migration and invasion in colorectal cancer by targeting AQP1.
MicroRNA-133a-3p inhibits cell proliferation, migration and invasion in colorectal cancer by targeting AQP1.
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DOI：
10.3892/ol.2021.12910
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发表时间：
2021-09
期刊：
影响因子：
2.9
通讯作者：
Wang B
中科院分区：
文献类型：
--
作者：
Kong B;Zhao S;Kang X;Wang B
最近，miR-133 a-3 p已被鉴定为人类结直肠癌（CRC）的标志物，并且miR-133 a-3 p与水通道蛋白1（AQP 1）之间的关联已在内皮细胞中被描述。然而，miR-133 a-3 p/AQP 1轴在CRC中的调节功能仍不清楚。本研究采用逆转录-定量PCR和western blot分析了miR-133 a-3 p和AQP 1在结直肠癌组织（n=56）和细胞系中的表达。采用χ2检验评估miR-133 a-3 p/AQP 1与结直肠癌患者临床病理特征的相关性。接下来，通过进行Cell Counting Kit-8和Transwell测定，在体外评估miR-133 a-3 p/AQP 1在CRC中的功能作用。此外，使用在线软件工具TargetScan7.1预测AQP 1作为miR-133 a-3 p的靶基因，随后使用荧光素酶报告基因测定进行验证。结果显示，与相应的对照组相比，miR-133 a-3 p在结直肠癌组织和细胞系中显著下调，而AQP 1则上调。临床研究表明，miR-133 a-3 p/AQP 1的表达与肿瘤TNM分期显著相关（P=0.020）。功能实验表明，miR-133 a-3 p过表达显著抑制CRC细胞的增殖、迁移和侵袭，而敲低miR-133 a-3 p则促进细胞增殖、迁移和侵袭。在CRC细胞中，AQP 1被鉴定和验证为miR-133 a-3 p的靶基因。大肠癌组织中AQP 1 mRNA表达水平与miR-133 a-3 p表达水平无相关性。此外，AQP 1敲低诱导miR-133 a-3 p对CRC细胞的抑制作用，而过表达逆转miR-133 a-3 p对CRC细胞的抑制作用。综上所述，这些发现表明miR-133 a-3 p可能通过靶向AQP 1抑制细胞增殖、迁移和侵袭而成为肿瘤抑制因子。
Recently, miR-133a-3p has been identified as a marker for human colorectal cancer (CRC) and the association between miR-133a-3p and aquaporin 1 (AQP1) has been described in endothelial cells. However, the regulatory functions of the miR-133a-3p/AQP1 axis remain unclear in CRC. The present study analyzed the expression of miR-133a-3p and AQP1 in CRC tissues (n=56) and cell lines using reverse transcription-quantitative PCR and western blot analysis. The χ2 test was used to assess the associations between miR-133a-3p/AQP1 and clinicopathological features of patients with CRC. Next, the functional role of miR-133a-3p/AQP1 in CRC was evaluated in vitro by performing Cell Counting Kit-8 and Transwell assays. Moreover, the online software tool TargetScan7.1 was used to predict AQP1 as the target gene of miR-133a-3p, followed by validation using a luciferase reporter assay. The results showed that miR-133a-3p was significantly downregulated, while AQP1 was upregulated in CRC tissues and cell lines compared with corresponding controls. Clinically, it was demonstrated that miR-133a-3p/AQP1 expression was significantly associated with tumor TNM stage (P=0.020). Functional experiments indicated that miR-133a-3p-overexpression remarkably suppressed, while knockdown promoted, cell proliferation, migration and invasion in CRC cells. Mechanically, AQP1 was identified and validated as a target gene of miR-133a-3p in CRC cells. The expression level of AQP1 mRNA was not correlated with miR-133a-3p expression in CRC tissues. Furthermore, AQP1-knockdown induced, while overexpression reversed, the suppressive effects of miR-133a-3p on CRC cells. Taken together, these findings suggested that miR-133a-3p might be a tumor suppressor by suppressing cell proliferation, migration and invasion via targeting AQP1.
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影响因子：
--
作者：
O'Shannessy DJ;Somers EB;Chandrasekaran LK;Nicolaides NC;Bordeaux J;Gustavson MD
通讯作者：
Gustavson MD
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DOI：
10.1186/s13046-018-0813-4
发表时间：
2018-07-18
期刊：
Journal of experimental & clinical cancer research : CR
影响因子：
--
作者：
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通讯作者：
Wa Q
影响因子：
--
作者：
Sugarbaker PH
通讯作者：
Sugarbaker PH
DOI：
10.1186/s13046-016-0310-6
发表时间：
2016-02-24
期刊：
Journal of experimental & clinical cancer research : CR
影响因子：
--
作者：
Dorward HS;Du A;Bruhn MA;Wrin J;Pei JV;Evdokiou A;Price TJ;Yool AJ;Hardingham JE
通讯作者：
Hardingham JE
甲基化介导的 miR-133a-3p 沉默通过 miR-133a-3p/MAML1/DNMT3A 正反馈环促进乳腺癌细胞迁移和干性。
DOI：
10.1186/s13046-019-1400-z
发表时间：
2019-10-28
影响因子：
11.3
作者：
Shi, Wanyue;Tang, Tingting;Pan, Yi
通讯作者：
Pan, Yi