Photobacterium profundum under pressure: a MS-based label-free quantitative proteomics study.
Photobacterium profundum under pressure: a MS-based label-free quantitative proteomics study.
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DOI：
10.1371/journal.pone.0060897
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Spagnolo L
中科院分区：
文献类型：
--
作者：
Le Bihan T;Rayner J;Roy MM;Spagnolo L
深光杆菌SS9是一种革兰氏阴性菌，最初采集自苏禄海。其基因组由两条染色体和一个80kb的质粒组成。虽然它可以在很宽的压力范围内生长，但P. profundum在28 MPa和15°C下生长最佳。它在大气压下生长的能力使其易于遗传操作和培养，使其成为研究精神病的模式生物。在这里，我们报告了使用无标记定量和质谱分析对在大气和高压下生长的P. profundum进行的鸟枪蛋白质组学分析。我们已经确定了与高压适应相关的差异表达蛋白，这些蛋白之前已经用其他方法报道过。参与关键代谢途径的蛋白质也被确定为差异表达。参与糖酵解/糖异生途径的蛋白质在高压下上调。相反，一些参与氧化磷酸化途径的蛋白在大气压下被上调。一些被区别识别的蛋白质被直接调节，以应对压力的物理影响。一些参与营养物质运输或同化的蛋白质的表达可能直接受到压力的调节。在自然环境中，不同的静水压力代表不同的生态系统，它们有自己特定的营养限制和丰度。然而，在这项研究中考虑的唯一变量是大气压力。
Photobacterium profundum SS9 is a Gram-negative bacterium, originally collected from the Sulu Sea. Its genome consists of two chromosomes and a 80 kb plasmid. Although it can grow under a wide range of pressures, P. profundum grows optimally at 28 MPa and 15°C. Its ability to grow at atmospheric pressure allows for both easy genetic manipulation and culture, making it a model organism to study piezophily. Here, we report a shotgun proteomic analysis of P. profundum grown at atmospheric compared to high pressure using label-free quantitation and mass spectrometry analysis. We have identified differentially expressed proteins involved in high pressure adaptation, which have been previously reported using other methods. Proteins involved in key metabolic pathways were also identified as being differentially expressed. Proteins involved in the glycolysis/gluconeogenesis pathway were up-regulated at high pressure. Conversely, several proteins involved in the oxidative phosphorylation pathway were up-regulated at atmospheric pressure. Some of the proteins that were differentially identified are regulated directly in response to the physical impact of pressure. The expression of some proteins involved in nutrient transport or assimilation, are likely to be directly regulated by pressure. In a natural environment, different hydrostatic pressures represent distinct ecosystems with their own particular nutrient limitations and abundances. However, the only variable considered in this study was atmospheric pressure.
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