Proteomic features predict seroreactivity against leptospiral antigens in leptospirosis patients.
Proteomic features predict seroreactivity against leptospiral antigens in leptospirosis patients.
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DOI：
10.1021/pr500718t
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发表时间：
2015-01-02
影响因子：
4.4
通讯作者：
Felgner, Philip L.
中科院分区：
文献类型：
--
作者：
Lessa-Aquino, Carolina;Wunder, Elsio A., Jr.;Lindow, Janet C.;Rodrigues, Camila B.;Pablo, Jozelyn;Nakajima, Rie;Jasinskas, Algis;Liang, Li;Reis, Mitermayer G.;Ko, Albert I.;Medeiros, Marco A.;Felgner, Philip L.
随着效率、准确性和速度的提高，我们可以从数千种感染性微生物中获得完整的基因组序列;然而，仍然需要仅基于氨基酸序列预测免疫系统抗原靶标的能力。在这里，我们使用了问号钩端螺旋体微阵列表达91%（3359）的所有钩端螺旋体预测的ORF（3667），并作出了经验会计的所有抗体反应性抗原，从自然感染的人血清中识别; 191抗原引起IgM或IgG反应，占整个蛋白质组的5%。我们将反应性抗原分类为26个注释的COG（邻苯二甲酸组簇），26个JCVI Mainrole注释和11个计算预测的蛋白质组学特征。总共鉴定了14个显著富集的类别，其与免疫识别相关，包括体外表达和体内mRNA上调的质谱证据。总之，这组14个丰富的类别只占25%的钩端螺旋体蛋白质组，但含有50%的免疫反应性抗原。这些发现与我们以前对其他革兰氏阴性菌的研究一致。这种全基因组方法提供了一个经验基础，预测和分类抗体反应性抗原的基础上，结构，物理化学和功能蛋白质组学特征和框架，了解免疫反应的广度和特异性L。质问者
With increasing efficiency, accuracy, and speed we can access complete genome sequences from thousands of infectious microorganisms; however, the ability to predict antigenic targets of the immune system based on amino acid sequence alone is still needed. Here we use a Leptospira interrogans microarray expressing 91% (3359) of all leptospiral predicted ORFs (3667) and make an empirical accounting of all antibody reactive antigens recognized in sera from naturally infected humans; 191 antigens elicited an IgM or IgG response, representing 5% of the whole proteome. We classified the reactive antigens into 26 annotated COGs (clusters of orthologous groups), 26 JCVI Mainrole annotations, and 11 computationally predicted proteomic features. Altogether, 14 significantly enriched categories were identified, which are associated with immune recognition including mass spectrometry evidence of in vitro expression and in vivo mRNA up-regulation. Together, this group of 14 enriched categories accounts for just 25% of the leptospiral proteome but contains 50% of the immunoreactive antigens. These findings are consistent with our previous studies of other Gram-negative bacteria. This genome-wide approach provides an empirical basis to predict and classify antibody reactive antigens based on structural, physical–chemical, and functional proteomic features and a framework for understanding the breadth and specificity of the immune response to L. interrogans.
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影响因子：
64.8
作者：
Malmström J;Beck M;Schmidt A;Lange V;Deutsch EW;Aebersold R
通讯作者：
Aebersold R
影响因子：
6.7
作者：
Caimano MJ;Sivasankaran SK;Allard A;Hurley D;Hokamp K;Grassmann AA;Hinton JC;Nally JE
通讯作者：
Nally JE
影响因子：
15.3
作者：
Noguchi, H
通讯作者：
Noguchi, H
影响因子：
--
作者：
Tan, Xiaolin;Chun, Sookhee;Davies, D. Huw
通讯作者：
Davies, D. Huw
影响因子：
7
作者：
Vigil, Adam;Chen, Chen;Felgner, Philip L.
通讯作者：
Felgner, Philip L.