Effect of renin gene transfer on blood pressure in the spontaneously hypertensive rat.
Effect of renin gene transfer on blood pressure in the spontaneously hypertensive rat.
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肾素基因转移对自发性高血压大鼠血压的影响。
DOI：
10.1161/01.hyp.31.1.373
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Pravenec,M
中科院分区：
文献类型：
--
作者：
StLezin,E;Liu,W;Wang,N;Wang,JM;Kren,V;Zidek,V;Zdobinska,M;Krenova,D;Bottger,A;vanZutphen,BF;Pravenec,M
为了研究是否在肾素基因的分子变异有助于更高的血压的自发性高血压大鼠（SHR）与血压正常的布朗挪威（BN）大鼠，我们测量血压在SHR的祖先株和SHR同源株是遗传上相同的，除了在肾素基因和相关的染色体13段从BN株转移。利用回交育种和分子选择技术，从血压正常的BN品系中获得了携带有肾素基因的SHR同系品系（命名为SHR.BN-Ren）。我们发现，从BN株转移到SHR的遗传背景的肾素基因并没有降低血压大鼠喂养正常或高盐饮食。事实上，SHR同类大鼠的收缩压往往略高于SHR祖大鼠的收缩压。然而，同源株表现出较低的血清高密度脂蛋白胆固醇，和更高水平的总胆固醇，极低密度脂蛋白，和中密度脂蛋白胆固醇在管理过程中的高脂肪，高胆固醇饮食。这些结果表明：（1）在本研究的环境条件下，SHR比BN大鼠的血压更高不能用肾素基因的品系差异来解释;（2）在转移的染色体片段内的13号染色体上存在影响脂质代谢的数量性状位点。SHR. BN-Rencongenic品系为研究高血压与血脂异常在心血管疾病中的相互作用提供了一种新的动物模型。
To investigate whether molecular variation in the renin gene contributes to the greater blood pressure of spontaneously hypertensive rats (SHR) versus normotensive Brown Norway (BN) rats, we measured blood pressure in an SHR progenitor strain and an SHR congenic strain that are genetically identical except at the renin gene and an associated segment of chromosome 13 transferred from the BN strain. Backcross breeding and molecular selection at the renin locus were used to create the SHR congenic strain (designated SHR.BN-Ren) that carries the renin gene transferred from the normotensive BN strain. We found that transfer of the renin gene from the BN strain onto the genetic background of the SHR did not decrease blood pressure in rats fed either a normal or high-salt diet. In fact, the systolic blood pressures of the SHR congenic rats tended to be slightly greater than the systolic blood pressures of the SHR progenitor rats. However, the congenic strain exhibited lower serum high-density lipoprotein cholesterol, and greater levels of total cholesterol, very-low-density lipoprotein, and intermediate-density lipoprotein cholesterol during administration of a high-fat, high-cholesterol diet. These findings demonstrate that (1) under the environmental circumstances of the current study, the greater blood pressure of SHR versus BN rats cannot be explained by strain differences in the renin gene and (2) a quantitative trait locus affecting lipid metabolism exists on chromosome 13 within the transferred chromosome segment. The SHR.BN-Rencongenic strain may provide a useful new animal model for studying the interaction between high blood pressure and dyslipidemia in cardiovascular disease.
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大鼠肾素基因：分配至 13 号染色体并与血压调节相关。
影响因子：
4.4
作者：
Pravenec,M;Simonet,L;Kren,V;Kunes,J;Levan,G;Szpirer,J;Szpirer,C;Kurtz,T
通讯作者：
Kurtz,T
Dahl SS/Jr 大鼠的基因污染。
DOI：
10.1161/01.hyp.23.6.786
发表时间：
1994
期刊：
Hypertension (Dallas, Tex. : 1979)
影响因子：
--
作者：
StLezin,EM;Pravenec,M;Wong,A;Wang,JM;Merriouns,T;Newton,S;Stec,DE;Roman,RJ;Lau,D;MorrisJr,RC
通讯作者：
MorrisJr,RC
由分配给大鼠 13 号染色体的多态性 DNA 标记定义的 9 个基因座的连锁图。
影响因子：
4.4
作者：
E. Remmers;E. Goldmuntz;H. Zha;P. Mathern;Y. Du;L. Crofford;R. Wilder
通讯作者：
R. Wilder
DOI：
10.1172/jci118230
发表时间：
1995
期刊：
The Journal of clinical investigation.
影响因子：
--
作者：
Purcell-Huynh,DA;Weinreb,A;Castellani,LW;Mehrabian,M;Doolittle,MH;Lusis,AJ
通讯作者：
Lusis,AJ
影响因子：
30.8
作者：
JACOB, HJ;BROWN, DM;LANDER, ES
通讯作者：
LANDER, ES