Primary structure and functional expression of a choline transporter expressed in the rat nervous system
Primary structure and functional expression of a choline transporter expressed in the rat nervous system
复制标题
大鼠神经系统中表达的胆碱转运蛋白的一级结构和功能表达
DOI：
10.1016/0014-5793(92)80649-2
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发表时间：
1992
期刊：
影响因子：
3.5
通讯作者：
H. Betz
中科院分区：
文献类型：
--
作者：
W. Mayser;P. Schloss;H. Betz
胆碱能神经末梢中神经递质乙酰胆碱的合成受质膜中钠驱动的高亲和力胆碱摄取系统调节。我们已经从大鼠脊髓和脑干中分离出编码胆碱转运蛋白（CHOT1）的cDNA。预测的蛋白质共享相当大的氨基酸身份和几个结构特征，包括12个推定的跨膜区与其他神经递质转运蛋白。体外转录的CHOT1 RNA在异种卵母细胞中的表达产生Na+依赖性胆碱摄取，这在对照卵母细胞中没有观察到。通过聚合酶链反应（PCR）扩增揭示了大量的CHOT1 mRNA在脑，小脑，脊髓，并在较小程度上，心脏，但只有非常低的表达在肺，肾和肌肉。
Synthesis of the neurotransmitter acetylcholine in cholinergic nerve terminals is regulated by a sodium‐driven high‐affinity choline uptake system in the plasma membrane. We have isolated cDNAs from rat spinal cord and brainstem which encode a choline transporter (CHOT1). The predicted protein shares considerable amino acid identity and several structural features including twelve putative transmembrane regions with other neurotransmitter transporters. Expression of in vitro transcribed CHOT1 RNA inXenopusoocytes generated Na+‐dependent choline uptake, which was not seen in control oocytes. Amplification by polymerase chain reaction (PCR) revealed significant amounts of CHOT1 mRNA in brain, cerebellum, spinal cord and, to a lesser extent, heart, but only very low expression in lung, kidney and muscle.
影响因子：
56.9
作者：
GUASTELLA, J;NELSON, N;KANNER, BI
通讯作者：
KANNER, BI
影响因子：
14.9
作者：
CAVENER, DR;RAY, SC
通讯作者：
RAY, SC