T lymphocytes in human atherosclerotic plaques are memory cells expressing CD45RO and the integrin VLA-1.
T lymphocytes in human atherosclerotic plaques are memory cells expressing CD45RO and the integrin VLA-1.
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人动脉粥样硬化斑块中的 T 淋巴细胞是表达 CD45RO 和整合素 VLA-1 的记忆细胞。
DOI：
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
G. Hansson
中科院分区：
文献类型：
--
作者：
S. Stemme;J. Holm;G. Hansson
近年来，一些免疫组织化学研究分析了人类动脉粥样硬化斑块的细胞组成。这些研究表明，斑块的主要细胞类型是巨噬细胞、平滑肌细胞和T淋巴细胞。为了进一步表征动脉粥样硬化斑块中的t淋巴细胞群，我们对人斑块组织进行酶解，并用荧光抗体标记释放的细胞，并通过流式细胞术进行分析。对15例行颈动脉内膜切除术的患者进行了研究。64%的斑块T细胞表达白细胞共同抗原（CD45）的低分子量形式（CD45RO）。这些细胞中有许多表达整合素极晚活化抗原-1 (vas -1)，这表明它们处于晚活化状态。相比之下，来自同一患者的外周血T细胞中只有1%表达vla1。T细胞活化的其他标志物，如Ta1 （CD26）和HLA-DR，在斑块T细胞上也增加。激活后短暂表达的白细胞介素-2受体（CD25）仅存在于一小部分细胞上。综上所述，对斑块淋巴细胞的分析表明，大多数斑块T细胞是记忆细胞，其中许多处于晚期或慢性激活状态。这种T细胞表型可能是活化的外周血T细胞优先募集和/或保留的结果，或者是静止T细胞局部抗原刺激的结果。
The cellular composition of human atherosclerotic plaques has been analyzed in several immunohistochemical studies in recent years. These studies have shown that the main cell types of the plaque are macrophages, smooth muscle cells, and T lymphocytes. To further characterize the T-lymphocyte population in atherosclerotic plaques, human plaque tissue was digested enzymatically and the released cells were labeled with fluorescent antibodies and analyzed by flow cytometry. Fifteen patients undergoing carotid endarterectomy were studied. Sixty-four percent of plaque T cells expressed the low-molecular-weight form (CD45RO) of the leukocyte common antigen (CD45). Many of these cells expressed the integrin very late activation antigen-1 (VLA-1), which suggests that they are in a state of late activation. In contrast, only 1% of peripheral blood T cells from the same patients expressed VLA-1. Other markers of T cell activation, such as Ta1 (CD26) and HLA-DR, were also increased on plaque T cells. The interleukin-2 receptor (CD25), which is transiently expressed after activation, was present on only a small proportion of the cells. Taken together, this analysis of plaque lymphocytes shows that the majority of plaque T cells are memory cells, many of which are in a state of late or chronic activation. This T-cell phenotype may be the result of a preferential recruitment and/or retention of activated peripheral blood T cells or local antigenic stimulation of resting T cells.
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影响因子：
20.1
作者：
HANSSON, GK;JONASSON, L;CLOWES, AW
通讯作者：
CLOWES, AW
人血管平滑肌细胞主要组织相容性基因表达的调节。
DOI：
10.1161/01.atv.9.3.279
发表时间：
1989
期刊：
Arteriosclerosis (Dallas, Tex.)
影响因子：
--
作者：
Warner,SJ;Friedman,GB;Libby,P
通讯作者：
Libby,P
影响因子：
4.4
作者：
通讯作者：
--
影响因子：
56.9
作者：
BEVILACQUA, MP;STENGELIN, S;SEED, B
通讯作者：
SEED, B
影响因子：
4.4
作者：
R. Rothlein;Michael Loran Dustin;S. D. Marlin;T. Springer
通讯作者：
R. Rothlein;Michael Loran Dustin;S. D. Marlin;T. Springer