A case study assessing the impact of mating frequency on the reproductive performance of the Hawaiian bobtail squid Euprymna scolopes.
A case study assessing the impact of mating frequency on the reproductive performance of the Hawaiian bobtail squid Euprymna scolopes.
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DOI：
10.1186/s42826-023-00168-1
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发表时间：
2023-07-28
影响因子：
2.9
通讯作者：
Miyashiro, Tim I.
中科院分区：
文献类型：
--
作者：
Cecere, Andrew G.;Cook, Rachel A.;Miyashiro, Tim I.
夏威夷短尾乌贼Euprymna nopes和细菌Vibrio fischeri之间的共生关系可以作为研究促进动物-细菌共生最初形成的分子机制的模型。该系统的研究经常依赖于新孵化的E。但在海水养殖设施中促进幼体生产的畜牧因素仍然报道不足。在这里，我们报告的繁殖性能E。交配频率降低的反应。一个动物队列在海水养殖设施中维持107天，雌性动物被分配到对照组（每14天交配一次）或实验组（每21天交配一次）。在存活率、产卵窝数或孵化数方面，未观察到组间差异。每组都有多只雌性过度繁殖，在人工饲养条件下，它们产卵量超过8窝。检查的分布，每日孵化计数的个别蛋离合器之间的离合器，是独立的交配频率的孵化模式显示显着的变化。最后，对孵化生产的评估表明，93.5%的总孵化所产生的队列来自蛋离合器奠定了在第一个70天。这些结果表明，较低的交配频率并不妨碍孵化生产。此外，不同卵窝孵化率的差异为研究E.作为微生物学研究的实验动物。在线版本包含补充材料，可通过10.1186/s42826-023-00168-1获得。
The symbiosis between the Hawaiian bobtail squid Euprymna scolopes and bacterium Vibrio fischeri serves as a model for investigating the molecular mechanisms that promote the initial formation of animal-bacterial symbioses. Research with this system frequently depends on freshly hatched E. scolopes, but the husbandry factors that promote hatchling production in a mariculture facility remain underreported. Here we report on the reproductive performance of E. scolopes in response to decreased mating frequency. One animal cohort was maintained in a mariculture facility for 107 days, with females assigned to either a control group (mating once every 14 days) or an experimental group (mating once every 21 days). No differences between the groups were observed in survival, the number of egg clutches laid, or hatchling counts. Each group featured multiple females that were hyper-reproductive, i.e., they generated more than 8 egg clutches while in captivity. Examination of the distributions for daily hatchling counts of individual egg clutches revealed significant variation in the hatching patterns among clutches that was independent of mating frequency. Finally, an assessment of hatchling production showed that 93.5% of total hatchlings produced by the cohort were derived from egg clutches laid within the first 70 days. These results suggest a lower mating frequency does not impede hatchling production. Furthermore, the variation in hatchling production among egg clutches provides new insight into the reproductive performance of E. scolopes as a lab animal for microbiology research. The online version contains supplementary material available at 10.1186/s42826-023-00168-1.
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影响因子：
4.9
作者：
Koch EJ;Miyashiro T;McFall-Ngai MJ;Ruby EG
通讯作者：
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2.9
作者：
Cecere, Andrew G.;Miyashiro, Tim I.
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Miyashiro, Tim I.
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